Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927370,0680AC58.3% 1.8 / 12.2 12V314G (GTA→GGA) thiPthiamine/thiamine pyrophosphate ABC transporter permease ThiP
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/7);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAAT  >  NZ_CP009273/69987‑70144
                                                                                 |                                                                            
gccgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg                                                                      >  1:99272/1‑90 (MQ=255)
          aaaTACGCAACGAGGCCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCg                                                            <  2:499459/89‑1 (MQ=255)
                                     cccACAGCCCGGGTGGTGCCACCACTCCCGGCAACGGGCGTTTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAAcagcagca                                 <  2:315290/89‑1 (MQ=255)
                                         caGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAgcgc                             >  2:168351/1‑90 (MQ=255)
                                         caGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAgcgc                             >  2:484199/1‑90 (MQ=255)
                                              ccGGTTGTGCCAGCCCTTCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca                        <  2:165168/90‑1 (MQ=255)
                                              ccGGTTGGCCCACCCCTCCCGGCACGTGGCATTTTCCCCCACCGACGATCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca                        <  1:105830/90‑1 (MQ=255)
                                               cGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca                        <  2:288787/89‑1 (MQ=255)
                                               cGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGca                        >  1:288787/1‑89 (MQ=255)
                                                ggTTGCCCCACCCCTCCCGGAACCGGGCGTTTCCCCCCACCGACGACCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcaca                      <  1:484199/90‑1 (MQ=255)
                                                         aGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACAcc             >  1:150913/1‑90 (MQ=255)
                                                                    aaaCGGGCGTTTCCCCCCCCCGACGTCCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAAt  <  1:302761/89‑1 (MQ=255)
                                                                                 |                                                                            
GCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAAT  >  NZ_CP009273/69987‑70144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑