Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,627	T→A	V84E (GTG→GAG)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,627	0	T	A	100.0%	28.0 / NA	9	V84E (GTG→GAG)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/5);  total (4/5)

GACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGC  >  NZ_CP009273/1613575‑1613709                                                     |                                                                                   gACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa                                               >  2:408984/1‑90 (MQ=255)        gAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGAGCGGCGTACTGGTaa                                        <  1:408984/90‑1 (MQ=255)                          tGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcg                      <  1:447869/90‑1 (MQ=255)                           ggATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgc                     >  2:186049/1‑90 (MQ=255)                               cGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCa                 <  1:368893/90‑1 (MQ=255)                                    ggTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtg            <  1:453574/90‑1 (MQ=255)                                              ggCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGc  >  2:30007/1‑90 (MQ=255)                                                 tGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCa                 <  1:105695/72‑1 (MQ=255)                                                 tGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCa                 >  2:105695/1‑72 (MQ=255)                                                     |                                                                                   GACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGC  >  NZ_CP009273/1613575‑1613709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑