| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 349,999 | 0 | T | G | 49.2% | 74.2 / 30.8 | 63 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (3/29); new base G (31/0); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC > NZ_CP009273/349911‑350088 | aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg < 2:358299/90‑1 (MQ=255) ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg > 1:457577/1‑90 (MQ=255) ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAg > 2:1175398/1‑90 (MQ=255) cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAgc > 1:998632/1‑90 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg < 1:650137/90‑1 (MQ=255) cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑g < 2:909746/90‑1 (MQ=255) cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCT‑g > 1:337110/1‑90 (MQ=255) tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑Gaa < 1:906980/90‑1 (MQ=255) ggggACGCGCGAGATTGAAGAGAGTATCTACAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGGGCT‑GAAAgggga > 1:292793/1‑88 (MQ=255) gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGGTGCCGAAGTGGCGGGGGGGGGGGGGGAAGAAGGGCTGGAAAgggag > 2:594801/1‑88 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCT‑GGAAAGGGAgggggg > 1:1203709/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGGGCT‑GAAAAGGGAgggggg > 1:603181/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGCGCT‑GAAAGGGCAgggggg > 2:673535/1‑89 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAgggggc > 1:35763/1‑90 (MQ=255) cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcggt < 1:594801/90‑1 (MQ=255) ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGt < 2:615546/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCT‑GGAAGGGGAGGGGGGGGGGGGGGTTGTCAt > 1:1094785/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGTTTGTGAt > 1:615466/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGGGGAAAATGGGCT‑GGAAGGGGAGGGGGGGGGGGGGGTTGtgtt > 2:807097/1‑87 (MQ=255) agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCC‑GAAAGGGGGGGGGGGGGGGGGGGTTGTCttt > 2:1094039/1‑90 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCC‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCCttt > 2:503041/1‑89 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCC‑GGAAGGGGAGGGGGGGGGGGGGGTTGTGATTc > 2:755376/1‑90 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGa > 1:462637/1‑90 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCT‑GAAAAGGAAGGGGGGGGGGGGGGTTTTCATTCCGa > 1:597050/1‑90 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag < 1:395250/90‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagag < 2:597050/90‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTCATTTCGAAagag > 2:315841/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCT‑GAAAGGGGGGGGGGGGGGGGGGTTTGTTATTCCGGAagag > 2:644896/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCT‑GGAAGGGGCGGGGGGGGGGGCGGTTGTCATTCCGAAagag > 2:1049713/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTCCTTCCCAAAGAGAGCg > 2:763821/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGGTTGTTATTCCGAAagaggggg > 1:202181/1‑86 (MQ=255) tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCT‑GGAAGGGGAGGGGGGGGGGGGGGTTGTCGTTCCCCAAAAGAGCgg > 2:1207625/1‑89 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:670489/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:315841/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:984240/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:793787/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:607582/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:1153057/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAAAGAGCGAc > 1:925746/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGAAAAAGAGCGAc > 2:244301/1‑90 (MQ=255) cagggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:117868/88‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 2:457577/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:1175398/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:810271/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:931651/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:736914/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAAAGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTTTTATTCCGAAAGAGGGGGACAg > 2:126024/1‑90 (MQ=255) cGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTGTTTCCGGAAAAGAGCGACAGTCt > 2:1070389/1‑90 (MQ=255) ttGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg < 1:673776/90‑1 (MQ=255) tGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCT‑GAAAAGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGGCAGGCTggg > 1:670489/1‑89 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa < 2:258585/90‑1 (MQ=255) aaGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAgctg > 1:117868/1‑87 (MQ=255) ggCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGGGACAGTCTGGGAGATCgggg > 2:913700/1‑87 (MQ=255) ggCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGGGGCAGGCTGGGAGATCGTGa > 2:190842/1‑90 (MQ=255) tGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 2:450637/90‑1 (MQ=255) tGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:551256/90‑1 (MQ=255) tGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:199303/90‑1 (MQ=255) tGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:190842/90‑1 (MQ=255) tGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:1143107/90‑1 (MQ=255) ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 2:615466/90‑1 (MQ=255) ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 2:454979/90‑1 (MQ=255) ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc > 1:454979/1‑90 (MQ=255) gggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt > 2:232098/1‑90 (MQ=255) tGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc < 1:244301/90‑1 (MQ=255) | AACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC > NZ_CP009273/349911‑350088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |