| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,979 | 0 | G | A | 47.8% | 11.8 / 13.6 | 23 | F80F (TTC→TTT) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (9/3); new base A (0/11); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAG > NZ_CP009273/1377907‑1378068 | gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACccgc > 2:488677/1‑90 (MQ=255) gCGACGTTTGTAATATCACGTCAAAACCTTCTTCTTCAGCTTGGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACccgccg < 2:132754/90‑1 (MQ=255) atCACGTCAAAACCTTCTTCTTCCGCTTGGGCGGTGATGGAATAAAAAATTAAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGaa < 2:1208083/90‑1 (MQ=255) accACGTCAAACCTTTCTTCTTCAGTTTGGGGGGTGATGGCATGAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGaa < 2:468849/88‑1 (MQ=255) accACGTCAAACCTTTCTTCTTCAGCTTTGGCGGTGATGGCATGAAAAACTTCAGAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGaa < 1:669087/88‑1 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAAc > 1:767499/1‑90 (MQ=255) cACGTCAAACCTTTCTTCTTCAGCTTGGGGGGTGATGGCATAAAAACCTTAAAAAAAAAAGGTATTCCCCGCCGTAGTTTTGGTCGAACg < 2:1057915/90‑1 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:809982/1‑90 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:785540/1‑90 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:576759/1‑90 (MQ=255) gTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGt > 1:1029663/1‑90 (MQ=255) aaCCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCTGTAGTTTTGGTCGAACGGGTAGaaa < 1:304589/90‑1 (MQ=255) ctttcttcttcAGCTTGGGGGGTGATGGCATGAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 1:534174/88‑1 (MQ=255) ccTTCTTCTTCATTTTTGGGGGTGATGGCATGAAAAACTTAAAAAAAAAACGGATCCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 1:785540/90‑1 (MQ=255) ccTTCTTCTTCAGTTTGGGGGGTGAGGGAATGAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 2:468033/90‑1 (MQ=255) ttcttcAGTTTTGGGGGTGATGGCATGCAAACCTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCAt < 1:771244/90‑1 (MQ=255) tcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGAt > 2:877540/1‑46 (MQ=255) tcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGAt < 1:877540/46‑1 (MQ=255) ggggTGAGGGCATAAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaaa < 1:1163776/88‑1 (MQ=255) ggTGATGGCATGAAAAACTTCAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAcc < 1:576759/90‑1 (MQ=255) gCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCa > 2:502652/1‑90 (MQ=255) ctacAGAAAAAACCGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcgcgc < 2:333954/87‑1 (MQ=255) tCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCgg > 1:333954/1‑90 (MQ=255) gaAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAg > 1:872647/1‑90 (MQ=255) | GTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAG > NZ_CP009273/1377907‑1378068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |