| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,561 | 0 | A | C | 60.0% | 9.7 / 13.7 | 15 | Y293S (TAC→TCC) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/9); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAA > NZ_CP009273/1673476‑1673648 | aTCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACcac < 1:626894/90‑1 (MQ=255) tgccAACTACGGCAACATGCCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAAAAAGGTGCTAAGTATTCCCACATCACCCGCCa < 1:1130150/87‑1 (MQ=255) cGGCAACATGCCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGGGGAGAAAGGTGCTAAGTTTTCCCACATCACCCGCCAGTGGCAGGa < 1:760658/90‑1 (MQ=255) aaaaTGACCGAAGCCTCCTATCAGGTTGCGAAACGTGCCGGGAAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGt < 1:917921/87‑1 (MQ=255) aaCATGACCGAAGTCTCCTTTCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTTTTCCCACACCACCCGCCAGTGGCAGGAACGt < 1:1125496/90‑1 (MQ=255) ctcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAAccctgt > 1:1105620/1‑86 (MQ=255) tATCGGGTTGCAAAACGTGCCGCGAAAAAAGGTGCTAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 1:1131213/90‑1 (MQ=255) tATCAGTTTGCAAAACGGCCCGCGAAGAAGGGTGCTAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 2:180688/90‑1 (MQ=255) aaaCGTGCCGCGAAAAAGGGTGCAAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTg < 1:1099188/90‑1 (MQ=255) ccGCGAAAAAGGGTGCTAATTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaat < 1:1139392/90‑1 (MQ=255) gCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTAc < 2:1080495/90‑1 (MQ=255) gCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACgg > 2:560242/1‑38 (MQ=255) gCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt < 1:560242/39‑1 (MQ=255) aaGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTcc > 1:1080495/1‑90 (MQ=255) ttACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATaa > 1:907039/1‑90 (MQ=255) accacaccacCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 2:859718/47‑1 (MQ=255) aCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc > 1:859718/1‑47 (MQ=255) | ATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAA > NZ_CP009273/1673476‑1673648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |