| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,900 | 0 | T | C | 52.9% | 7.0 / 20.8 | 17 | G71G (GGA→GGG) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base C (0/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCG > NZ_CP009273/2356820‑2356988 | ttGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCccg > 1:153031/1‑90 (MQ=255) ggCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAAc > 1:584270/1‑90 (MQ=255) cTTCATGCACCCCCGGCCTTTCCCTTTTTGCTGCCGCGGGGGCGAGTGCGCTAAAATCACCGCTGGTCGGCCCCAGCCCGCCGTTAACGa < 2:312857/90‑1 (MQ=255) cTTCAGGCACCACCGGGCCTTCCCTTTTTGCTGCCGCGGGGGCGGGGGCTTTAAAATCATCGCGGGTCGGCCCCACCCCGCCGTTAACGa < 2:913839/90‑1 (MQ=255) tCAGGCACCCCCAGCCCTTCCCTTTTTGCTGCCGCGGGGGGGGGGGCGCTAAAATCACCGCTGGTCGGCCCCAGCCCGCCGTTAACGATc < 2:140681/90‑1 (MQ=255) agcaCCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGcaca > 1:268493/1‑90 (MQ=255) gcaCCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACAt > 1:312857/1‑90 (MQ=255) gcaCCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACAt > 1:959453/1‑90 (MQ=255) ggcccTTCCCTTTTTGCTGCCGCGGGGGCGGGGGCGTTAAAACCACCGCGGGGCGGCCCCACCCCGCCGTTAACGATCAGCACATCGGCg < 2:153031/87‑1 (MQ=255) aGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCg > 1:371938/1‑90 (MQ=255) gcccTTCCCTTTTTCCGGCCGCGGGGGCGAGGGCCTTAAAATCATCGCGGGTCGGCCCCACCCCGCCGTTAACGATCAGCACATCGGCGt < 2:161157/88‑1 (MQ=255) ttttGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACg > 2:578155/1‑90 (MQ=255) gCTGCCGCAGCGGCGGGTGCGTTTAAACCATCGCTGGTCGGCCCCACCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTcg < 1:581174/90‑1 (MQ=255) gTGCGCTAAAATCATCGCGGGCCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTa < 1:1129558/90‑1 (MQ=255) tGCGTTAAAACCCTCGCTGGTCGCCCCCACCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTaa < 1:578155/90‑1 (MQ=255) aaaaTCATCGTGGGCCGGCCCCCGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcatc < 1:699554/89‑1 (MQ=255) catcGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGt > 2:568457/1‑90 (MQ=255) ggtcggccCCCCCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATcccc < 2:230496/90‑1 (MQ=255) gtcCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCg > 2:390056/1‑90 (MQ=255) | TTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCG > NZ_CP009273/2356820‑2356988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |