| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,571,502 | 0 | A | C | 57.1% | 9.0 / 16.6 | 14 | L79V (TTA→GTA) | maeB | NADP‑dependent oxaloacetate‑decarboxylating malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTC > NZ_CP009273/2571415‑2571586 | cAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAAcc > 1:983586/1‑90 (MQ=255) aaaTACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAAccc > 1:168344/1‑90 (MQ=255) ccATCACCCCCGGCGATTTTCTAAAACAGACCCCCCTTCCCTCCCACCCCCGGTTTC‑CCTG‑CCAGCGCGCCAATGTTGCCTACCCCCAGc < 2:759357/89‑1 (MQ=255) aaTCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAACCCCAGCAccgc > 1:93471/1‑90 (MQ=255) cccGGCGATCTCCTAAAACACACCCCCCTTGCCTCCCCCCCCGGGTCCG‑CCGC‑CCGGCGCGCCAATGTTCCCTACCCCCAGCCCCGCCGt < 2:130834/90‑1 (MQ=255) aaaCAGAACGCCCTTGCTTTCCCCCCCCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTCCCAACCCCCAGCACCGCCGTACCGTTAGAGATCAc < 1:873334/90‑1 (MQ=255) aaCGCCCTTGCCTTCCATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGccac > 2:873334/1‑90 (MQ=255) ccATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCggg > 1:12805/1‑90 (MQ=255) caccACCGTTTTC‑CCTC‑CCGGCCCCCAATTTTTCCCTACCCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc < 1:1100867/87‑1 (MQ=255) ccccccGGTTTGCCCCC‑CCACCGCCCCAATTTCCCCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc < 1:777431/86‑1 (MQ=255) caccccGGTTTT‑CCCGCCCAGCGCCCAAATTTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc < 1:314858/86‑1 (MQ=255) g‑cctc‑ccGGCGCGCCATTGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTa < 1:496936/90‑1 (MQ=255) cccgg‑ccAGCGCGCAAATTTTCCCCACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTa < 1:831301/86‑1 (MQ=255) gCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTc > 1:711537/1‑90 (MQ=255) | CAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTC > NZ_CP009273/2571415‑2571586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |