Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,898 G→A A26T (GCC→ACC)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,8980GA94.7% 116.6 / ‑2.2 38A26T (GCC→ACC) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (14/22);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49820‑49982
                                                                              |                                                                                    
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGcc                                                                           <  2:105020/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGcc                                                                           <  2:909430/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGcc                                                                           <  1:602278/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGcc                                                                           <  1:693318/90‑1 (MQ=255)
       tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTa                                                                    <  1:845040/90‑1 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTaa                                                                   >  2:134912/1‑90 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCGTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  2:595400/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  2:839155/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  2:681330/90‑1 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                       >  2:860355/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCtt                                                       >  1:1177631/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCtt                                                       >  1:329571/1‑90 (MQ=255)
                         tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaata                                                  <  2:953326/90‑1 (MQ=255)
                                aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                           <  1:605721/90‑1 (MQ=255)
                                 gATCGCGTTATCGGCATGGAAAACGCCAAGCCGTGGAACCTGCCTACCGATCTCGCCGGGGTTAAACGCAACACCTTAAATAAACCCGTg                                          >  2:1081670/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                        <  2:632019/72‑1 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                        >  1:632019/1‑72 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                      >  2:336933/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAAAGCAACACCTTAAATAAACCCGTGATTa                                      >  2:549274/1‑90 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                   <  1:860355/90‑1 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                        <  1:119477/69‑1 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAAAGCAACACCt                                                        >  2:119477/1‑69 (MQ=255)
                                          aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                 <  1:444288/90‑1 (MQ=255)
                                           tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                <  2:522911/90‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                              <  2:728147/87‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                              >  1:728147/1‑87 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                           <  2:87295/90‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                           <  2:35095/90‑1 (MQ=255)
                                                    aaaaCGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                       <  1:409418/90‑1 (MQ=255)
                                                        cGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   >  1:110892/1‑90 (MQ=255)
                                                        cGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   >  2:612616/1‑90 (MQ=255)
                                                                 gTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                 <  2:191387/67‑1 (MQ=255)
                                                                 gTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                 >  1:191387/1‑67 (MQ=255)
                                                                 gTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg          >  2:46396/1‑90 (MQ=255)
                                                                   ggAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                          >  2:955582/1‑72 (MQ=255)
                                                                   ggAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                          <  1:955582/72‑1 (MQ=255)
                                                                   ggAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc        <  2:110892/90‑1 (MQ=255)
                                                                   ggAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc        <  1:167139/90‑1 (MQ=255)
                                                                         tgcctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt  >  1:647831/1‑90 (MQ=255)
                                                                              |                                                                                    
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49820‑49982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: