| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 481,482 | T→G | L89* (TTA→TGA) | acrR → | multidrug efflux transporter transcriptional repressor AcrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 481,482 | 0 | T | G | 97.1% | 130.4 / ‑3.8 | 34 | L89* (TTA→TGA) | acrR | multidrug efflux transporter transcriptional repressor AcrR |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (19/14); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATTAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGC > NZ_CP009273/481395‑481567 | gTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATTaa > 2:228776/1‑90 (MQ=255)gTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGaa > 1:767296/1‑90 (MQ=255) gagaTCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAaga < 1:918347/90‑1 (MQ=255) cTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAAtt > 1:25978/1‑90 (MQ=255) tGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTc > 1:766111/1‑90 (MQ=255) gTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCa > 2:258602/1‑90 (MQ=255) tCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCAt < 1:331348/90‑1 (MQ=255) aaTATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGaa > 1:322202/1‑90 (MQ=255) atatTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAAt < 1:363709/90‑1 (MQ=255) atTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATcc < 1:1032102/90‑1 (MQ=255) ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAagagag > 1:683300/1‑63 (MQ=255) ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAagagag < 2:683300/63‑1 (MQ=255) ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACg > 2:404851/1‑90 (MQ=255) ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACg > 2:310159/1‑90 (MQ=255) ggTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACg > 2:643187/1‑90 (MQ=255) gAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTg < 1:310574/90‑1 (MQ=255) gAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTg < 1:780158/90‑1 (MQ=255) aCTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGAc < 1:455733/90‑1 (MQ=255) agagCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACaga < 1:643187/90‑1 (MQ=255) ttGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAAc > 2:683991/1‑90 (MQ=255) ttGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAAc > 2:1163283/1‑90 (MQ=255) ttGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAAc > 2:1005638/1‑90 (MQ=255) gAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACgg < 2:799221/90‑1 (MQ=255) gAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACgg > 2:94749/1‑90 (MQ=255) aGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGc > 1:252742/1‑90 (MQ=255) gTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCg < 1:1016013/90‑1 (MQ=255) tCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCg > 1:1150344/1‑90 (MQ=255) ttCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATg < 2:216782/90‑1 (MQ=255) ttCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATg < 1:374429/90‑1 (MQ=255) ttCCCTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATg < 1:404851/90‑1 (MQ=255) ccTGGCGATCCACTCTCAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATGgag > 1:41345/1‑90 (MQ=255) cAGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAAt > 1:20578/1‑90 (MQ=255) aGTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATg > 2:296047/1‑90 (MQ=255) gTATGAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGc > 2:465358/1‑90 (MQ=255) | GTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTAGAGCTTGAGTATCAGGCAAAATTCCCTGGCGATCCACTCTCAGTATTAAGAGAGATATTAATTCATGTTCTTGAATCCACGGTGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGC > NZ_CP009273/481395‑481567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |