| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,371,314 | 0 | G | A | 47.2% | 22.6 / 19.4 | 36 | F475F (TTC→TTT) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase |
| Reads supporting (aligned to +/- strand): ref base G (14/5); new base A (0/17); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA > NZ_CP009273/2371232‑2371393 | tCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAAttt > 2:758871/1‑90 (MQ=255) ggcgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCccgcc < 1:563301/90‑1 (MQ=255) gTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgtt > 1:1711978/1‑90 (MQ=255) cctaaaaaGGGACGTTTTGCGGCATAAAAAAAAACCCCCCCCGCTCGTTTTGGGGCGTTGGCAACAGGGAAAAAATTTGCCCGCCgttgt < 2:1479097/86‑1 (MQ=255) ggggcGTTTTGCGGCATAAGATAGAACCGCCCCCGCTCGTTTTGCGGCGTGGGAAACAGCAAAAAATTTTGCCCGCCGTTGTTGTTcacc < 2:1812564/86‑1 (MQ=255) ggccGTTTGGCGGCATAAGAAAGAACCCCCCCCGCTCGTTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcacca < 2:37120/87‑1 (MQ=255) ggccGTTTGGCGGAATCAGAAAAAAACCCTCACGCTCGTTTTGCGGCGTTGGAAACAGCGAAAAATTTTCCCCGCCGTTGTTGTTcacca < 2:293407/87‑1 (MQ=255) ggAGGTTTTGCGGCATCAGATAGAACCGCTCCCCCTCGCTTTGCGGGGTTGGCAAAAGCGAAAAAATTTGCCCGCCGTTGTTGTTcacca < 1:1349373/90‑1 (MQ=255) ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca < 1:303280/90‑1 (MQ=255) gACTTTTTGCGGCATAAGATAGAACCCCCCCCCCTCGTTTTGCGGCGTGGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 2:1711978/90‑1 (MQ=255) gACGTTTTGCGGCATCAGATAGAAACGCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAAAAAATTTCCCCGCCGTTGTTGTTcaccac < 1:2252080/90‑1 (MQ=255) gACGTTTTGCGGCATCAGATAAAACCGCTCCCGCTCGCTTTGCGGCGTTGGAAAAAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 1:1293333/90‑1 (MQ=255) gACGTTTTGCGGCATAAGATAAAACCCCCCCCCCTCGTTTTGCGGCGTTGGCAACAGCGAAAAATTTCGCCCGCCGTTGTTGTTcaccac < 2:1817135/90‑1 (MQ=255) gACGTTTTGCGGAATAAGATAAAACCGCTCCCCCTCGTTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 2:643924/90‑1 (MQ=255) aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgc > 2:353170/1‑90 (MQ=255) gATAAAACCGCTCCCGCCCCCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcggcg < 1:1802395/90‑1 (MQ=255) gAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgtt < 1:512/57‑1 (MQ=255) gAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgtt > 2:512/1‑57 (MQ=255) gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCt > 1:392195/1‑90 (MQ=255) gctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCt > 1:1624862/1‑90 (MQ=255) ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCTGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTg > 2:202162/1‑90 (MQ=255) ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcaccac > 2:1307683/1‑63 (MQ=255) ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcaccac < 1:1307683/63‑1 (MQ=255) ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTg > 2:1891072/1‑90 (MQ=255) cccgctcgttTTGCGGGGTTGCCAACAGGGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGAc < 2:40124/88‑1 (MQ=255) ccgctcgttTGGGGGTGTGGGCAACAGGGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACg < 2:227422/89‑1 (MQ=255) ccgctcgttTGGGGGCGTGGCAAACAGCGAAAAATTTCCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACg < 2:2263806/89‑1 (MQ=255) cgctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGc > 1:659594/1‑90 (MQ=255) ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAAc > 1:227422/1‑90 (MQ=255) ggggcgTGGGAAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa < 1:1501119/88‑1 (MQ=255) gcggcgTTGGCAACAGCGAGAGAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa > 1:310976/1‑90 (MQ=255) ggcgTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgc < 1:322372/90‑1 (MQ=255) cgTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc > 2:320352/1‑90 (MQ=255) ttGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt > 1:1147262/1‑90 (MQ=255) ggccAACAGCGAAAAATTTTCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt < 2:2133427/87‑1 (MQ=255) gCAACAGCGAAAAAATTCCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga < 2:1549095/90‑1 (MQ=255) | TCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA > NZ_CP009273/2371232‑2371393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |