| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 61.9% | ‑6.0 / 21.6 | 21 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (1/7); new base A (13/0); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGA > NZ_CP009273/2380262‑2380422 | ggCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGcac < 2:1687145/90‑1 (MQ=255) cTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACat < 1:1662959/90‑1 (MQ=255) gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTccc < 1:923485/90‑1 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACATGCACAATTTTTTTTTCCTcct > 1:405067/1‑88 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTcct > 1:824881/1‑88 (MQ=255) tgtTATACCTTTGTTATCCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑c < 2:1454876/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCCGAAAAAAAGAAAAGCAAAATTTTTTTTTCCTCCT‑CTT‑TTTTTAg > 2:503491/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTATTTTTCCTCCT‑CTG‑tttttaa > 1:919985/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGGGCTGCAAAATTTTTTTTTTCCCCT‑CTG‑tttttaa > 1:1455589/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCT‑CTG‑tttttaa > 1:717551/1‑87 (MQ=255) ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCAT‑CTG‑TTTTTAAATGCGa > 1:120157/1‑90 (MQ=255) taCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTG‑TTTTAAGATGCGaa < 1:1998839/90‑1 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAAAAAAGCACATTTTTTTTTTTCTCAT‑CTG‑TTTTTTAATGCGaaaaaa > 2:1998839/1‑87 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACAATTTTTTTTTCCTCCTCCTT‑TTTTAAAAGGGGaaaaaga > 1:1636643/1‑86 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCCGAAAAAACGACAAGCACATTTTTTTTTTTCTCCT‑CTT‑TTTTTATATGGGGAACAAGaaaataa > 2:550314/1‑86 (MQ=255) taataaAAATTTCTCGAGGCGGGCACCGAAAAAACGACAAGGAAATTTTTTTTTTTTTCCT‑CTGTTTTTAAGATG‑GAAAAAAGaaaataa > 2:1211845/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTATTTTTCCTCAT‑CTG‑TTTTTAAATGCGAAAAATGAAAATCAAc > 1:1034376/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGGCCCGAAAAAAGGAAAGGCACATTTTTTTTTTCCTCTT‑CTG‑TTTTTAAATGGGAAAAAAGAAATTTaaa > 1:1845404/1‑89 (MQ=255) aGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaa < 2:1636643/90‑1 (MQ=255) ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaat < 2:919985/90‑1 (MQ=255) cAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGa > 1:231221/1‑90 (MQ=255) | GGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAT‑CTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGA > NZ_CP009273/2380262‑2380422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |