| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 46.8% | 72.4 / 31.1 | 62 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (8/25); new base T (29/0); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGC > NZ_CP009273/3175881‑3176044 | gAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑CCACGTTTTTTTGCGCGttttt > 2:1865910/1‑88 (MQ=255) aGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑CCCCCCTTTTTTGCGCGTTtttcc > 1:1255344/1‑89 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑CCCCGTTTTTTTGGGCGGTTTTCCGCGt > 2:859994/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGCG‑CCCCGTTTTTTTGCGCGTTTTTCcgcgg > 1:1166848/1‑89 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCCCGTTTTTTTGCGCGTTTTTCCGCGt > 1:673825/1‑90 (MQ=255) aaGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCCTTTTTTTGGGGGGTTTTTC‑GCGGTCTC‑ggggca > 2:737050/1‑87 (MQ=255) ggAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCaaaa < 1:687201/90‑1 (MQ=255) ggAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCaaaa < 2:382184/90‑1 (MQ=255) ggAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCaaaa < 1:191568/90‑1 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑CCACGTTTTTTTGCGCGGTTTTTCGCGTTCTC‑CGGCaaaaa > 2:646338/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑CCCCGGTTTTTTGGGCGGTTTTCTGCGTTCTT‑CGGGAAAAAcc > 2:1054190/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCCGTTTTTTGG‑GCGTTTTTTGGCGTTTTT‑GGGGaaaaaac > 2:1829186/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAG‑CCACGGTTTTTGGCGCGTTTTTCGGCGCTCTC‑CGGCCAAAAcc > 1:1921444/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCGG‑CCGCGTTTTTTGGCGCGGTTTTTTGCGGTCTC‑TGGCaaaaaac > 2:1787607/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCGG‑CCACGTTTTTTGGCGCGTTTTTCTGCGGTCTC‑GGGCAAAAAcc > 1:1371874/1‑90 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑GCCCCTTTTTTTGGGCGTTTTTTTGCGCTGTCGGGGCAAAAAccc > 2:1660691/1‑90 (MQ=255) aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAAc < 2:420381/90‑1 (MQ=255) tCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAG‑CCACGTTTTTTTGGGCGTTTTTCGGCGTTCTC‑CGGCAAAAAACCCCCAAcccc > 2:53932/1‑88 (MQ=255) tCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGc > 1:863447/1‑90 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGGAG‑CCACGGTTTTTTGGGCGGTTTTCCGCGGTCTC‑CGGGAAAAAACCCCCAACCCCTTa > 2:575181/1‑90 (MQ=255) ccTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTg < 1:575181/90‑1 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGGG‑CCACCCTTTTTTGGGCGGTTCTCGGCGGTCTC‑GGGCAAAAAACCCTGAACCCCTTAATGGc > 1:276648/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAG‑CCACGTTTTTTGGGGCGTTTTTCGGCGTTTTC‑GGGCCAAAAACCCCGAACCGCTTTCTGGc > 1:396029/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAG‑CCACGGTTATTTGGGGGGTTTTCCGCGTTTTC‑GGGCAAAAAACCCCGAACCGCTTCCTggg > 1:1868904/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAG‑CCACGCTTTTTTGGGCGTTTCTCTGCGGTCTC‑GGGCCAAAAACCCCCAACCCCCTACTGGc > 1:2199321/1‑90 (MQ=255) ctcATTCTGACCATACTGGCGGGGGGAG‑CCACGTTTTTTTGCGCGTTTTTCGGCGGTCTC‑CGGCCAAAAACCCCCAACCCCCTACTGGCg > 1:2036046/1‑90 (MQ=255) cATTCTGACCATACTGGCGGGGGGAG‑CCCCGGTTTTTTGCGCGGTTTTCGGCGGTCTC‑CGGGAAAAAACCCCGCACCCCGTTCTGGCggt > 2:442150/1‑88 (MQ=255) ttCTGACCATACTGGCGGGGGCAG‑CCACGTTTTTTTGGGCGGTTTTCGGCGGTTTC‑GGGCAAAAAACCCCGAACCGCTTTCTTGCGgttt > 1:2001291/1‑90 (MQ=255) ttCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGtttt < 2:1265402/90‑1 (MQ=255) gACCATACTGGCGGGGGGGG‑CCACGTTTTTTTGGGCGTTTTTCTGCGTTTTC‑GGGCGAAAAACCCCCAACCGCTTACTGGGGGTTTTGCt > 2:1784363/1‑90 (MQ=255) gACCATACTGGCGGGGGGAG‑CCACGTTTTTTTGGGCGTTTCTCCGCGTTCTC‑CGGCCAAAAACCCCGCACCCCCTTCTGGCGgtttttct > 2:924760/1‑86 (MQ=255) gACCATACTGGCGGGGGCAG‑CCCCGTTTTTTTGCGCGTTTTTCGGCGTTTTC‑GGGCAAAAAACCTCGAACCGCTTTCTGGCGGTTTTGCt > 1:935800/1‑90 (MQ=255) gACCATACTGGCGGGGGCAG‑CCCCGGTTTTTTGCGCGGTTTTCGGCGTTCTC‑CGGCAAAAAACCCCGAACCCCTTTCTTGCGGTTTTGCt > 2:86053/1‑90 (MQ=255) aCCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 1:53932/90‑1 (MQ=255) aCCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 2:2199321/90‑1 (MQ=255) aTACTGGCGGGGGGAG‑CCACGTTTTTTTGCGCGTTTTTCCGCGTTTTC‑CGGCAAAAAACCCCGAACCCCTTTCTGGGGGTTTTGCTAggg > 1:2216297/1‑89 (MQ=255) aTACTGGCGGGGGCAG‑CCACGTTTTTTGGCGCGTTTTTCGGCGTTCTC‑GGGCAAAAACCCCCGAACCCCTTACTGGCGGTTTCGCTAGGt > 1:267636/1‑90 (MQ=255) tACTGGCGGGGGCAG‑CCCCGGTTTTTTGCGCGGTTTTCCGCGTTCTC‑CGGGAAAAAACCCCGAACCGCCTTCTTGCGGTTTTGCTAgggt > 2:412844/1‑88 (MQ=255) tGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTg < 1:787142/90‑1 (MQ=255) gCGGGGGGAG‑CCACGTTTTTTTGCGGGTTTTTCCGCGGTCTC‑GGGCAAAAACCCCCCAACCCCCTTCTGGCGGTTTTGCTAGGGTTTgtg > 2:1030261/1‑88 (MQ=255) gCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCCGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcg > 1:71478/1‑90 (MQ=255) cGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcgg < 1:833434/90‑1 (MQ=255) gggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggc < 2:1255344/90‑1 (MQ=255) ggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:2215582/90‑1 (MQ=255) ggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:863447/90‑1 (MQ=255) ggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:771527/90‑1 (MQ=255) ggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:1660691/90‑1 (MQ=255) ggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:465781/90‑1 (MQ=255) ggggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:1030261/90‑1 (MQ=255) gggCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGcggcgg < 2:2064612/90‑1 (MQ=255) aG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:824036/90‑1 (MQ=255) aG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:312179/90‑1 (MQ=255) aG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:1509617/90‑1 (MQ=255) aG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 2:1166848/90‑1 (MQ=255) aG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:1163164/90‑1 (MQ=255) g‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCggggggc > 1:2214869/1‑87 (MQ=255) g‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCgggggac > 2:1507178/1‑87 (MQ=255) g‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGTc > 1:852472/1‑90 (MQ=255) cGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGt > 1:153756/1‑90 (MQ=255) gTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCaaaa < 1:1935084/36‑1 (MQ=255) gTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCaaaa > 2:1935084/1‑36 (MQ=255) ttATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGc < 1:1954483/90‑1 (MQ=255) | GAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGC > NZ_CP009273/3175881‑3176044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |