| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,169 | 0 | T | G | 46.0% | 60.8 / 23.5 | 50 | V252G (GTG→GGG) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (1/26); new base G (23/0); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGC > NZ_CP009273/937080‑937257 | tGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGt < 2:873071/90‑1 (MQ=255)tGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGt < 2:1211581/90‑1 (MQ=255)tGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGt < 1:1773447/90‑1 (MQ=255) ggCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTg < 2:1531828/90‑1 (MQ=255) gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGa < 2:70665/90‑1 (MQ=255) gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGa < 1:1774280/90‑1 (MQ=255) cAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAc > 1:252531/1‑90 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATACCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 2:1542738/90‑1 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 1:1606819/90‑1 (MQ=255) gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACTTACt > 1:325331/1‑90 (MQ=255) cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATc > 1:1349125/1‑90 (MQ=255) tCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTGCTATCTTTaaaaag > 2:2049708/1‑86 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGc < 1:1799550/90‑1 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGc < 1:1366849/90‑1 (MQ=255) aCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGcc < 2:433665/90‑1 (MQ=255) aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAg < 2:1349125/90‑1 (MQ=255) gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACTTACTATCTTGAACAGGCACGCCAGa > 1:2051089/1‑90 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCTTTAAAAAGGACGCCCGAAAACTaa > 2:254140/1‑90 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCAc < 2:1996292/76‑1 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCAc > 1:1996292/1‑76 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCTTTAAAAGGCACGCCCGAAAACTAAt > 2:569910/1‑90 (MQ=255) aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTa < 2:422066/60‑1 (MQ=255) aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTa > 1:422066/1‑60 (MQ=255) gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCAt < 2:1525550/90‑1 (MQ=255) tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATTTTTGACAGGGACCCCCGAAAACTAATTCCCCCATg > 1:978954/1‑90 (MQ=255) tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGATTTTTATCTTTAACAAGGGCGCCCGAAAATTAATGCCCCCATg > 2:1437084/1‑90 (MQ=255) ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGa < 1:569910/90‑1 (MQ=255) ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatc < 1:1890230/90‑1 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcatc < 1:1249459/90‑1 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCCGAAAACTAATGCCCGCATGatcatcatc > 1:2196993/1‑90 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcatc > 1:565066/1‑90 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGatcatcatc > 1:2045595/1‑90 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 1:854282/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 1:532126/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 1:1737621/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 2:2177725/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAAGCACGCCAGAAAACTAATTCCCGCCTGATCATCATTGATc > 2:854282/1‑90 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTGAAAAGGCACGCCAGAAAACTAAAGCCCGCATGATCATCATCGATc > 1:1928367/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTa < 1:1547084/39‑1 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAAGGACGCCAGAAAACTAATGGCCCCATGATCATCATCGATcc > 2:763896/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATcc > 2:302834/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACCCCAGAAAATTAATGCCCCCATGATCATCatccatcc > 1:1332692/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTa > 2:1547084/1‑39 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATcc > 1:769951/1‑90 (MQ=255) gCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcgc < 1:1876452/90‑1 (MQ=255) aaaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt < 1:2049708/90‑1 (MQ=255) aaaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt < 1:2199580/90‑1 (MQ=255) aCGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGGACCCCAGAAAACTAATGCCCCCATGATCATCATCGATCCGCGCtat > 1:1879541/1‑90 (MQ=255) gAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg < 2:769951/90‑1 (MQ=255) ggTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGcc > 1:810168/1‑90 (MQ=255) tGGGGGGACTTTCTATCTTTAACAGGGACGCCAGAAAACTAATGCCCCCCTGATCATCATCGATCCGCGCCATAACCACACCCGGGCCgg > 2:567136/1‑90 (MQ=255) gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcgc < 2:537893/90‑1 (MQ=255) gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcgc < 1:407352/90‑1 (MQ=255) | TGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGC > NZ_CP009273/937080‑937257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |