| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 60.0% | 7.7 / 17.5 | 20 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (12/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC > NZ_CP009273/822875‑823037 | aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggggggg > 2:90700/1‑88 (MQ=255) gtgggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGC‑AACGCCGGa > 1:39264/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCCGAAg > 1:171044/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGG‑AAAGCCGGGAg > 2:94051/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGC‑GACGCCGGAAg > 2:307555/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATACACGGCGCGGGTGGGGGGCGAACGCCGGag > 1:472865/1‑89 (MQ=255) ggtggtGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGCGGGGGGG‑CACGCCCGAAga > 2:281926/1‑89 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCga < 2:26308/90‑1 (MQ=255) ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGaa < 1:9203/90‑1 (MQ=255) ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGaa < 1:178478/90‑1 (MQ=255) gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGG‑AAAGCCCGAAGGCGGGGCTTCGGGAAACAg > 2:321721/1‑90 (MQ=255) gCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGC‑AACGCCGGAAGGCGGGTCGGCGAGAAAAAGAAt > 1:256200/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:256200/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGC‑AACGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGc > 1:456881/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGC‑AACGCCCGAAGTCGGTTTGTCGAGAAAAAGAATGTCCGGGTCATgg > 1:305632/1‑89 (MQ=255) tGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGC‑AACGCCGGGAGTCGGTTCGTCGGGAAAAAAAAAGTGCGGGTCATGCa > 2:172034/1‑90 (MQ=255) gtgCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACa < 2:71737/90‑1 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGG‑AAAGCCGGGAGGCGGGTTGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 2:168680/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGC‑AACGCCGGAAGTCGGGTCGTCGGGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgg > 1:480957/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGTGAGGGGGGC‑AACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGgcc > 1:61835/1‑90 (MQ=255) cggcggGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:281926/90‑1 (MQ=255) | ATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTC‑AACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC > NZ_CP009273/822875‑823037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |