Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,257,2980TG58.3% 2.1 / 12.6 12T12P (ACC→CCC) prsribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAAAACTGTGC  >  NZ_CP009273/1257222‑1257386
                                                                            |                                                                                        
tCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCa                                                                             >  1:289726/1‑90 (MQ=255)
tCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTGTGCTAGTTTCGGGGGGGGGGTTCCAGCa                                                                             >  2:87724/1‑90 (MQ=255)
          gACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGGTACCAGCAAAAAGCTTCa                                                                   >  2:11500/1‑90 (MQ=255)
           aCCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCAAAAAACTTCat                                                                  >  1:338251/1‑90 (MQ=255)
                cAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCAAAAAGCTTCATATCAg                                                             >  1:170839/1‑90 (MQ=255)
                     gcgTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGGGGGGGTACCAGCAAAAAACTTCATATCAGGCACg                                                        >  2:120903/1‑90 (MQ=255)
                                acaggtgtACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGATCCTc                                             <  2:371378/86‑1 (MQ=255)
                                              ggTTGGCAATACGTTGTGCTAGTTCCGGGGGGGCGTTACCAGCAAAAAACTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAt                               >  1:238535/1‑90 (MQ=255)
                                                ttGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                             <  1:138440/90‑1 (MQ=255)
                                                           ttGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt                  >  2:437616/1‑90 (MQ=255)
                                                                          ggTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAAAACtgtg   <  1:398661/90‑1 (MQ=255)
                                                                           gTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAAAACTGTGc  <  1:432190/90‑1 (MQ=255)
                                                                            |                                                                                        
TCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAAAACTGTGC  >  NZ_CP009273/1257222‑1257386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑