Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,453,6300CG60.0% 1.2 / 10.2 10A107G (GCG→GGG) paaC3‑hydroxyacyl‑CoA dehydrogenase PaaC
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base G (0/6);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 4.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAA  >  NZ_CP009273/1453553‑1453686
                                                                              |                                                        
gcTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGccc                                              >  1:134873/1‑90 (MQ=255)
                 tgttCATGGAGGCGGCGTCTGACCGTC‑GGAAAAAAAAAAAGCCGCTCTTCCCACGGGGGGGGGATTTTTCCCCGCCACAAACGCTATTGa                             <  2:341063/87‑1 (MQ=255)
                 tGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGa                             >  1:386004/1‑90 (MQ=255)
                    tCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCa                          >  2:103546/1‑90 (MQ=255)
                             cggcgTCGGAACGTCAGAAAAAAAAAAAAG‑CCCTTTTTGCACGGGGGGGGGATTTTTCCCCGCCACAAACGCTATTGACCACTAACACtt                 <  2:134873/90‑1 (MQ=255)
                                   ctcaaCGTGTGAAAGAAAAAAACG‑CCCTCTTCCCACGGGTGGGGGAATTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCaa           <  1:49932/87‑1 (MQ=255)
                                   cTGAACGTCTGAAAAAAAAAAAGG‑CTCTTTTTGCCCGGGGGGGGGATGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCaa           <  1:276249/90‑1 (MQ=255)
                                   cTGAACGTCGGAAAGAAAAAAAAG‑CCCTTTTTGCACGGCGGGGGGAATTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCaa           <  1:164452/90‑1 (MQ=255)
                                         gTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCta     >  1:196687/1‑90 (MQ=255)
                                            gggAAGAAAAAAAAG‑CGCTCTTTCCACAGCGGGGGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATaa  <  1:103546/89‑1 (MQ=255)
                                                                              |                                                        
GCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAG‑CGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAA  >  NZ_CP009273/1453553‑1453686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑