| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,294,154 | 0 | G | T | 64.3% | ‑2.9 / 13.0 | 14 | T161N (ACC→AAC) | ubiT | SCP2 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base T (0/9); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGG‑TTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCC > NZ_CP009273/3294066‑3294225 | gcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGG‑TTTCAGGCGCGGt > 1:384224/1‑90 (MQ=255) aaTACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGG‑TTTCAGGCGCGGTTTTCATTccc > 1:421512/1‑90 (MQ=255) cGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGG‑TTTCAGGCGCGGTTTTCATTCCCGCCTca > 1:402696/1‑90 (MQ=255) cATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGG‑TTTCAGGCGCGGTTTTCATTCCCGCCTCAACaaa > 1:321906/1‑90 (MQ=255) ttttCTCCTCAAATTAGCTTGTTCCCCCTCCGGTTTTTTTTTTGT‑TTTCGGGCGGGTTTTTCATCCCCGCCTCAACAAAATCCGCCAGtt < 2:477319/89‑1 (MQ=255) tccTCGAATTAGCGTGTTCCCCCTCCCGATGTTTGTTTGT‑TTTCGGGCGCGTTTTTAATCCCCGCCTCAACAAAATCCGCCAGTTgcagc < 2:209386/88‑1 (MQ=255) aaTTAGCTTGTTCCCCCTACCGTTTTTTTTTTGT‑TTTCGGGCGGGTTTTTCATCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatc < 2:227251/90‑1 (MQ=255) tgtttCACCTCCCGTTGTTTGTTTGG‑TTTAAGGCGGGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTaa < 1:123334/87‑1 (MQ=255) cTACAGTTGTTTGTTTGT‑TTTCGGGCGGGTTTTTCTTCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCg < 2:353350/90‑1 (MQ=255) tttttGGTTTTCGGGCGCGTTTTTCATCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTc < 2:321906/88‑1 (MQ=255) tttttGG‑TTTCGGGCGCGGTTTTCATCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc < 1:405769/88‑1 (MQ=255) tgtttgt‑tTTCAGGCGGGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc < 1:315566/90‑1 (MQ=255) tgtttgt‑tTTCAGGCGGGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc < 1:461144/90‑1 (MQ=255) tgtttgt‑tTCCGGGGGGGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTcc < 2:252282/90‑1 (MQ=255) | GCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGG‑TTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCC > NZ_CP009273/3294066‑3294225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |