Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,368,6020GT54.5% 1.5 / 10.7 11P54T (CCT→ACT) ampCBlaEC family class C beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACA  >  NZ_CP009273/4368539‑4368681
                                                               |                                                                                
acGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTaccgccaccg                                                       >  2:404086/1‑90 (MQ=255)
           gtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCggg                                            >  1:420051/1‑90 (MQ=255)
               ggggCTTTTTTTGGGGGATGCCCGCATAGCCCCGGGAAAAG‑AAAAAGGTTTTCCCGTAAAAATTTCCCGCCACCGCCATACCCGGGATCt                                        <  1:418908/89‑1 (MQ=255)
                                aTGCCCGCATCCCCCAAGGAAAAGTAATAAGTTTTCCCC‑TAAAAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc                       <  1:788/90‑1 (MQ=255)
                                aTGCCCGCATCCCCCAAGGAAAAAAAAAAAGTTTTCCCC‑TGAAAAATCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc                       <  1:254385/90‑1 (MQ=255)
                                aTGCCCGCATACCCCCAGGAAAAGTAATAAGTTTTCCCC‑TAATAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc                       <  1:153965/90‑1 (MQ=255)
                                aTGCCCGCATA‑CCCCAAGAAAAAAAAAAAGGTTTTCCCTTAAAAAATCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc                       <  2:108390/90‑1 (MQ=255)
                                  gcccGCAAACCCCAAGAAAAAGAAATAAGTTTTCCCC‑TAAAAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCgg                     <  2:158368/88‑1 (MQ=255)
                                               aaGGAAAAGAAATAAGTTTTCCCC‑TAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGa        <  1:26485/89‑1 (MQ=255)
                                                    aaaGTAAAAAGTTTTCCCC‑TAATAAATTCCCCCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcac   <  1:14174/90‑1 (MQ=255)
                                                     aaGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcaca  >  2:239286/1‑90 (MQ=255)
                                                               |                                                                                
ACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACA  >  NZ_CP009273/4368539‑4368681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑