| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,368,611 | 0 | G | A | 57.1% | 4.8 / 13.1 | 14 | Q51* (CAG→TAG) | ampC | BlaEC family class C beta‑lactamase |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base A (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAG > NZ_CP009273/4368539‑4368700 | acGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTaccgccaccg > 2:404086/1‑90 (MQ=255) gtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCggg > 1:420051/1‑90 (MQ=255) ggggCTTTTTTTGGGGGATGCCCGCATAGCCCCGGGAAAAG‑AAAAAGGTTTTCCCGTAAAAATTTCCCGCCACCGCCATACCCGGGATCt < 1:418908/89‑1 (MQ=255) aTGCCCGCATCCCCCAAGGAAAAGTAATAAGTTTTCCCC‑TAAAAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 1:788/90‑1 (MQ=255) aTGCCCGCATCCCCCAAGGAAAAAAAAAAAGTTTTCCCC‑TGAAAAATCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 1:254385/90‑1 (MQ=255) aTGCCCGCATACCCCCAGGAAAAGTAATAAGTTTTCCCC‑TAATAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 1:153965/90‑1 (MQ=255) aTGCCCGCATA‑CCCCAAGAAAAAAAAAAAGGTTTTCCCTTAAAAAATCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 2:108390/90‑1 (MQ=255) gcccGCAAACCCCAAGAAAAAGAAATAAGTTTTCCCC‑TAAAAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCgg < 2:158368/88‑1 (MQ=255) aaGGAAAAGAAATAAGTTTTCCCC‑TAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGa < 1:26485/89‑1 (MQ=255) aaaGTAAAAAGTTTTCCCC‑TAATAAATTCCCCCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcac < 1:14174/90‑1 (MQ=255) aaGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcaca > 2:239286/1‑90 (MQ=255) ttcccc‑TAAAAAATTCCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt < 1:373665/87‑1 (MQ=255) ttACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt > 1:12480/1‑90 (MQ=255) ttACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt > 1:493817/1‑90 (MQ=255) gATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAg > 1:152308/1‑90 (MQ=255) | ACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAG > NZ_CP009273/4368539‑4368700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |