Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,152,7270TG54.5% 9.9 / 13.4 11G165G (GGT→GGG) ycfHmetal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCG  >  NZ_CP009273/1152663‑1152808
                                                                |                                                                                 
aagaaAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACa                                                          <  2:400091/90‑1 (MQ=255)
          gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATGGATTTCCGGTTTTAAATCTCCttttt                                                >  2:522103/1‑89 (MQ=255)
                                  aggTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGc                        <  1:124988/88‑1 (MQ=255)
                                              ggACAGAGAAACGGCGGGGAAATTACTGGGTTTCGGGTTTTAAATCTCCTTTTTCGGCCTTGTGGCCTTCCCTAATTCGGGGCAAATgcg            >  2:14348/1‑90 (MQ=255)
                                              ggACAGAGAAACGGCGGGGAAATTACTGGATTTCGGGTTTTAAAATTCCTTTTTCGGCATTTTGGCCCTCCCGAATGCGGGGGAACTgcg            >  2:64499/1‑90 (MQ=255)
                                              ggACAGAGAAACGGCGGGGAAATTAATGGGTCTCGGATTTTTCATCTCCTTTTTCGGCCTTTTGGCCTTCCCGAATGCGGGGGAACTgct            >  2:124988/1‑89 (MQ=255)
                                              ggACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTTAATCTCCCTTTTCGGCCTTTTGACCCTTCCTAAAGCGGGGGAACcgcg            >  2:214745/1‑90 (MQ=255)
                                                   gagaAACGGCGGGGAAAATACTTGATCTCGGGTTTTATATCTCCTTTTTCCGCAGTGTGAGCTTCCCCAATGCGGGGGAAATGCGCGGtg       >  1:385036/1‑90 (MQ=255)
                                                     gaAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgct     <  1:64499/90‑1 (MQ=255)
                                                        aCGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTgcg  <  2:151057/90‑1 (MQ=255)
                                                        aCGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTgcg  <  2:385036/90‑1 (MQ=255)
                                                                |                                                                                 
AAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCG  >  NZ_CP009273/1152663‑1152808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑