| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,927,661 | 0 | A | T | 55.6% | 3.5 / 23.3 | 18 | S401T (TCA→ACA) | edd | phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base T (0/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTG > NZ_CP009273/1927573‑1927741 | gCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGAt > 2:328255/1‑90 (MQ=255)gCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGAt > 2:19737/1‑90 (MQ=255) aGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGcc < 1:328255/90‑1 (MQ=255) ccGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 2:577574/1‑90 (MQ=255) ccGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 2:124878/1‑90 (MQ=255) taaaCATTTTTGCCCCCCCATGAGGAGAAAAAGGTTTTTCGAAGGAAGCGATCACTTTGCTGTCGAGTGTTTTTCCCCCCCCTTCCCGcc < 1:19737/88‑1 (MQ=255) tgccccACCATGATGAGAAAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTGGAGTGTTTTTTCCCCCCCTCCCCGCCAGTCCAGTTc < 2:402967/87‑1 (MQ=255) atgatgAGAAAAAGTTTGTTCGAAGGAAGCGATCACTTTGTTGTCGAGTGTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCattatt < 1:242047/90‑1 (MQ=255) gatgaGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCa > 2:582614/1‑90 (MQ=255) gagaAGGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:189686/90‑1 (MQ=255) gagaAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTTTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:43050/90‑1 (MQ=255) tttgtcCAAAGGACGCGACCACCTTGCTCTGAGGTTTTTTTCCCCCCCCTGCCCGCCAGTCCAGTTCACCATTCTTCAGCCATGGTTCaa < 1:452086/89‑1 (MQ=255) gttgttCGAAGGAAGCGATCACTTTGCTGTGGAGTTTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCaa < 2:582761/90‑1 (MQ=255) gttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg > 1:180937/1‑90 (MQ=255) ttCGAAGGAAGCGACCACTTTGTTGTGGGTTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 1:280446/90‑1 (MQ=255) tccaaaggaagCGATCACATGGTGGTCGAGTTTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 1:297380/86‑1 (MQ=255) cGATCACTTTGCTGTCGATTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagaca < 1:124878/90‑1 (MQ=255) tGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTg > 1:490907/1‑90 (MQ=255) | GCACGGCCCAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTG > NZ_CP009273/1927573‑1927741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |