| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,927,669 | 0 | G | C | 64.7% | 3.5 / 17.5 | 17 | A398G (GCG→GGG) | edd | phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base C (0/11); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.70e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTAT > NZ_CP009273/1927582‑1927750 | aGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGcc < 1:328255/90‑1 (MQ=255) ccGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 2:577574/1‑90 (MQ=255) ccGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 2:124878/1‑90 (MQ=255) taaaCATTTTTGCCCCCCCATGAGGAGAAAAAGGTTTTTCGAAGGAAGCGATCACTTTGCTGTCGAGTGTTTTTCCCCCCCCTTCCCGcc < 1:19737/88‑1 (MQ=255) tgccccACCATGATGAGAAAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTGGAGTGTTTTTTCCCCCCCTCCCCGCCAGTCCAGTTc < 2:402967/87‑1 (MQ=255) atgatgAGAAAAAGTTTGTTCGAAGGAAGCGATCACTTTGTTGTCGAGTGTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCattatt < 1:242047/90‑1 (MQ=255) gatgaGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCa > 2:582614/1‑90 (MQ=255) gagaAGGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:189686/90‑1 (MQ=255) gagaAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTTTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:43050/90‑1 (MQ=255) tttgtcCAAAGGACGCGACCACCTTGCTCTGAGGTTTTTTTCCCCCCCCTGCCCGCCAGTCCAGTTCACCATTCTTCAGCCATGGTTCaa < 1:452086/89‑1 (MQ=255) gttgttCGAAGGAAGCGATCACTTTGCTGTGGAGTTTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCaa < 2:582761/90‑1 (MQ=255) gttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg > 1:180937/1‑90 (MQ=255) ttCGAAGGAAGCGACCACTTTGTTGTGGGTTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 1:280446/90‑1 (MQ=255) tccaaaggaagCGATCACATGGTGGTCGAGTTTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 1:297380/86‑1 (MQ=255) cGATCACTTTGCTGTCGATTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagaca < 1:124878/90‑1 (MQ=255) tGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTg > 1:490907/1‑90 (MQ=255) ttttttCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTAt < 1:276938/89‑1 (MQ=255) | AGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTAT > NZ_CP009273/1927582‑1927750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |