| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,388,923 | 0 | A | C | 54.2% | 24.1 / 28.5 | 24 | G151G (GGT→GGG) | nuoJ | NADH‑quinone oxidoreductase subunit J |
| Reads supporting (aligned to +/- strand): ref base A (7/4); new base C (0/13); total (7/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.53e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGC > NZ_CP009273/2388846‑2389010 | ttcGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGagcagc < 2:540595/90‑1 (MQ=255) ccaTTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGGGGAAGG‑CCACCACCACACCTGCGAGCAGCAGCATAGAAGCCAGGTcc > 1:377143/3‑90 (MQ=255) cGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTcc > 2:173775/1‑90 (MQ=255) cGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTcc > 2:339473/1‑90 (MQ=255) cAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGagcagc < 2:26129/64‑1 (MQ=255) cAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGagcagc > 1:26129/1‑64 (MQ=255) tcccccccCACGCTCTTCACGCCCGACGTGGAAGG‑CCACAACCAGCCCTGCGAGCAGCAGCATAGAAGCCAGTCCCACCGCCAGTACGTa < 2:522647/86‑1 (MQ=255) ccgcccgcTCTCCACGCCCGACGGGGAAGC‑CCACAACCAGCCCGGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGcc < 1:173775/90‑1 (MQ=255) ccctctCCCCGCCCGACGGGAAAGC‑CCAAAACCAGCCCGGCGAGCAGCAGAAAAAAAGCCATTTCCACCGCCAGTACGTAAGGCCCGAAc < 1:262995/88‑1 (MQ=255) cGCTTTTCACGCCCGCCGGGGAAGC‑CCACACCCAGCCCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAAc < 2:454685/90‑1 (MQ=255) cGCTCTTCACGCCCGACGTGGAAGG‑CCACAACCAGCCCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAAc < 2:282072/90‑1 (MQ=255) cGCTCTTCACGCCCGA‑GGGGAAGGCCCACAACCAGCCCGGGGAGAAGAAGAATAAAAGCCATTTCCACCGCCAGTACGTAAGGCCCGAAc < 1:393117/90‑1 (MQ=255) cGCTCTTCACGACCGACGGGGAAGG‑CCACACCCAGCCCGGCGAGCAGCAGCATAAAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAAc < 1:38859/90‑1 (MQ=255) cACGCCCGACGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTaa < 2:138470/90‑1 (MQ=255) cACGCCCGACGGGGAAGG‑CCACAACCAGCCCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTaa < 2:523032/90‑1 (MQ=255) aaCGCCCGCCGGGGAAGC‑CCACACCCAGCCCGGCGAGCAGCAGAAAAAAACCCATTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTaa < 1:272205/89‑1 (MQ=255) ccgacGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATAccc > 2:32168/1‑90 (MQ=255) gacGTGGAAGG‑CCACACCCAGCCCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCAc < 2:247457/90‑1 (MQ=255) gacGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGcc > 1:443653/1‑58 (MQ=255) gacGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGcc < 2:443653/58‑1 (MQ=255) ccGTGGAAGC‑CCACAACCAGCCCGGCGAGCAGCAACATAAAAGCCATTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACt < 1:2075/89‑1 (MQ=255) acGTGGAGGC‑CCACAACCAGCCCGGCGAGCAGCAGCAAAAAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACt < 1:141278/90‑1 (MQ=255) acGGGGAAGG‑CCAAAACCAGCCCTGCGAGCAGCAGCATAAAAGCCAGTCCCCCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACt < 2:470623/90‑1 (MQ=255) aGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGc > 1:280055/1‑90 (MQ=255) | TTCGCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGG‑CCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGC > NZ_CP009273/2388846‑2389010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |