| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 69,900 | 0 | A | C | 56.2% | 10.4 / 17.4 | 16 | V370G (GTG→GGG) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease ThiP |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAA > NZ_CP009273/69815‑69979 | gATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTg > 2:3963/1‑90 (MQ=255) gttgttGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGc > 2:387586/1‑90 (MQ=255) aGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTc > 2:591669/1‑90 (MQ=255) ccGGTACCCAGCCCACCCCCGGGCAGGGGGAGGATCAACATGCCCCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGc < 1:497054/90‑1 (MQ=255) caATCCCCGGCATGGCGGGGACAAACATGCCCCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGc < 1:581405/90‑1 (MQ=255) aTCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTc > 2:205933/1‑90 (MQ=255) ggCAGGGGGAGGATAAACATGCCGCCCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagc < 2:519978/90‑1 (MQ=255) tGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCCCCCGCAGTTCGCGACTGCTCCATAgcagca > 1:222329/1‑90 (MQ=255) ggAGGATCAACATGCCGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcatca < 1:209100/89‑1 (MQ=255) aTCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTc < 2:189400/90‑1 (MQ=255) cAACATCCCCCTCATCCCCACCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg < 1:3963/90‑1 (MQ=255) tcccGCTCACCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTa < 1:222889/88‑1 (MQ=255) tGCCGCTCACCTCCAGCCCCTGCCCTGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTa < 1:222888/90‑1 (MQ=255) ccGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACg < 1:247676/90‑1 (MQ=255) cGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc < 2:167472/90‑1 (MQ=255) cATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGGCAGCACTACGCACaa > 2:310293/1‑90 (MQ=255) | GATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAA > NZ_CP009273/69815‑69979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |