| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,348,816 | 0 | A | C | 56.2% | 10.8 / 16.5 | 16 | T245P (ACC→CCC) | gltB | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/9); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAAC > NZ_CP009273/3348748‑3348905 | ccGTCCCGCGCGGGCCGCTGGCGCACCCGTTCCGCTTTCTGGCGCATAACGGTAAAATCACCCCCATCCCCGGTAACCGCCAATGGgcgc < 2:378594/90‑1 (MQ=255) cGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcg > 2:281623/1‑90 (MQ=255) cgcgCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgc > 1:89274/1‑90 (MQ=255) cgctggccgctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgcgt > 1:518704/1‑90 (MQ=255) gggcccgcgggcGCACCCTTTCCGTTATTGGGGGCATAAGGGAAAAACCAACCCCATCACGGGAACCCGCCAATGGGCGCGTGCGCGTAc < 2:518704/86‑1 (MQ=255) ctggccgctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTAc > 1:545520/1‑90 (MQ=255) ggccgctggcGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACtt > 1:189419/1‑90 (MQ=255) cgctggcGCACCCGTTCCGCTATCTGGCGCATAACGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTtata < 2:213243/90‑1 (MQ=255) cgctggcGCACCCGTCCCGCTTTCTGGCGCATAACGGTAAAATCAACCCCCTCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTtata < 2:474157/90‑1 (MQ=255) tcgggCGCATAACGGTAAAACCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 2:189419/87‑1 (MQ=255) tCTGGCGCATAAGGGTAAAAACACCCCCATCCCGGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 2:308326/90‑1 (MQ=255) ggCGAATAACGGTAAAACCACCCCCATCCCCGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGa < 2:491262/90‑1 (MQ=255) aagggTAAAACCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 2:545520/87‑1 (MQ=255) aaCGGTAAAATCAACCCCACCCCCGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 1:16175/90‑1 (MQ=255) aaCGGTAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCac < 2:173864/90‑1 (MQ=255) taaaaaCAACCCCACCCCCGATAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGAcgc < 1:281623/88‑1 (MQ=255) aCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAAc > 1:440731/1‑90 (MQ=255) | CCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAAC > NZ_CP009273/3348748‑3348905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |