| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,578,446 | 0 | A | C | 56.2% | 7.4 / 19.9 | 16 | Y580D (TAC→GAC) | ggt | gamma‑glutamyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCC > NZ_CP009273/3578359‑3578533 | cTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcc > 1:130283/1‑90 (MQ=255) aCAGAATTACAAAAAAAAAAAGG‑CTCCCTTCGGCTTCCCCTAAAAAAATACCCCTCTCCCCACGAAGAGGCCCGCTAACCTTAGTCcccc < 1:133837/90‑1 (MQ=255) ttACGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt > 1:206033/1‑90 (MQ=255) aCGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTaa > 2:143193/1‑90 (MQ=255) aCGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTaa > 2:60899/1‑90 (MQ=255) aaaaaaaaaaAGGG‑CTCCCTCCGGCTTCCCCTGAAAAAATACCCCTCTCCCCACGAAGGGGCCCGCTACCCTTAGTCCCCCGCCGTTaaa < 2:206033/88‑1 (MQ=255) aaaaaaGGCCTCCCTTGGCCTTCCCCTAAAAAAACCCCCCCCCCCCCACGAGGGGGCCCCCTACCTTTAGCCCCCCGCCGTTAAatcatc < 1:337888/90‑1 (MQ=255) cctcccTCCGCCTTCCCCTAAAAAAACCCCCCCCTCCCCACGAGGGGGCCCGCTACCTTTATCCCCCCGCCGTTAAATCATCCACCGAgc < 1:67073/86‑1 (MQ=255) tACCTCCGCCTCCCCCTAAAAAAATCCCCCTCTCCCCAAGAGGGGGGCCCCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAgcgc < 1:217544/90‑1 (MQ=255) tccccTGAAAAAATACCCCTCTCCCCACGAAGGGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc < 2:141683/88‑1 (MQ=255) cccTAACAAAATCCCCCCCCCCCCCCGAGGGGGGCCGCTACCCTTACCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgcgc < 1:263051/90‑1 (MQ=255) cccTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcccc > 1:401328/1‑39 (MQ=255) cccTCTCCCCACGAAGAGGGCCGCTAACCTTAGTCcccc < 2:401328/39‑1 (MQ=255) aCGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCaa > 1:584205/1‑90 (MQ=255) gggcccGCTACCCTTACCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATaa < 1:216456/86‑1 (MQ=255) gTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGcc > 2:29303/1‑90 (MQ=255) | CTACAGGATTACGAAATAAAAAAGG‑CTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCC > NZ_CP009273/3578359‑3578533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |