| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,464,506 | 0 | A | G | 61.1% | 2.4 / 25.7 | 37 | E189G (GAA→GGA) | bdcR | TetR/AcrR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (8/6); new base G (22/0); total (31/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAG > NZ_CP009273/4464422‑4464587 | gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGa > 1:59729/1‑90 (MQ=255)gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGa > 1:367644/1‑90 (MQ=255) gTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaa > 2:474066/1‑90 (MQ=255) gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGGTAAAAAatat > 1:499138/1‑90 (MQ=255) gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAt < 2:147222/90‑1 (MQ=255) tGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGGGCGATGAAAACAAttgt > 1:560773/1‑88 (MQ=255) tGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAAAACAACTCTGTGCCAGAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCt < 1:344390/90‑1 (MQ=255) ttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTgtgga > 1:487966/1‑90 (MQ=255) gggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGAccc > 2:259024/1‑90 (MQ=255) gggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTTTTGAGGGGGGATGCGGGCCTTGAccc > 2:250587/1‑90 (MQ=255) tctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTGGGGGGGGGGCGGGGCTTGGCCCGAAA‑gg > 1:269420/1‑90 (MQ=255) gAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGTGAAAAACTTTTCTTTAGGAGCGGTTCGGGCCGTGAACCAAAA‑AGCGGGaa > 2:259134/1‑90 (MQ=255) gAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGGGGGATGCGGGCCTTGACCCGAAA‑GGCgggga > 2:531728/1‑88 (MQ=255) caacTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTCTTTAGGAGGGATGCGGGCCTTGTCCCGAAA‑AGGGGGGAAgg > 1:507462/1‑89 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTTTTGAGGGGGGAAGCGGGGCTTGACCCCAAA‑GGGGGGGAAacgc > 1:513703/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCCAAA‑AGCGGGGAAgcgc > 1:41638/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGGGATGCGGGCCTTGACCCGAAA‑GGCGGGGAAacgc > 1:108869/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGAGATGCGGGCCTTTACCCGAAA‑GGCGGGGAAacgc > 1:594081/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGTGATGCGGGCCTTTACCCGAAA‑GGCGGGGAAacgc > 2:459735/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAA‑GGCGGGGAAgcgc > 1:198618/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAA‑GGCGGGGAAacgc > 1:128628/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAACATTCTTTGGGAGGGATGCGGGCCGTGACCCGAAA‑GGCGGGGAAgggc > 1:574077/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAt < 1:11836/45‑1 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAt > 2:11836/1‑45 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAgcgc > 2:149095/1‑90 (MQ=255) ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGcc < 1:250587/90‑1 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGGGGGAAAACGCCt > 2:146314/1‑90 (MQ=255) tctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGCCt > 2:53911/1‑90 (MQ=255) tgtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGCCTGc < 2:499138/90‑1 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAAAATTTTTGAGGGGGGATGCGGGCCTTGGCCCGAAAGGGGGGGAAAGGGccgcc > 1:71626/1‑87 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGGGGGGAAGCGCCTGcc > 2:269501/1‑90 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGCAGGGATAAAAACTAATCTTGAGGGGCGATGCGGGGCTTGACCCGAAA‑GGCGGGAAAGcgcccgcc > 2:227659/1‑90 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGCCTGcc > 2:31810/1‑90 (MQ=255) gCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGGCTTGGCCCGAAA‑GGGGGGGAAAAGCCTGCCGGTAAGtt > 1:289548/1‑90 (MQ=255) gCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGCCTGCCGATAAggt > 1:171456/1‑88 (MQ=255) tGCAATGGCGGGGGAAGCGATAAAAACTATTCTTTAGGGGTGATGCGGGCCTTTACCCGAAA‑GGCGGGAAAGCGCCCGCCCATAAATtgt > 2:268151/1‑90 (MQ=255) ggCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGCCTTTGACCCGAAA‑GGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAg < 2:560773/90‑1 (MQ=255) | GTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAA‑GGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAG > NZ_CP009273/4464422‑4464587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |