| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,013 | 0 | A | C | 57.1% | 11.2 / 17.2 | 14 | intergenic (+128/+5) | gsk/ybaL | inosine/guanosine kinase/Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTC > NZ_CP009273/496935‑497089 | tttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc < 1:572589/89‑1 (MQ=255)tttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc > 2:572589/1‑89 (MQ=255) gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgc > 1:395689/1‑90 (MQ=255) gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc > 2:201254/1‑90 (MQ=255) tCATCCCTCCTTTTTTTTCATTTTTTCCCCCCGCCACCCCCCTTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 1:304083/90‑1 (MQ=255) tCATCCCTAATTTTTTTTCATTTTTAACCCCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 1:25586/90‑1 (MQ=255) tCATCCCTAAATTTTTTTCATTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 1:66253/90‑1 (MQ=255) ccATCCCTACATTTTTTTCATTTTTTCCACCCGCCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 1:156212/89‑1 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 2:289122/1‑90 (MQ=255) ccTACATATTTTTCATTTTTTACACCCGGCAACCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 2:245377/90‑1 (MQ=255) aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt > 1:245377/1‑90 (MQ=255) attttttACATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt < 2:344431/87‑1 (MQ=255) tACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt < 2:395689/90‑1 (MQ=255) acacCCGCCACCCCCCGTTCCCCCCCCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTc < 1:289122/90‑1 (MQ=255) | TTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTC > NZ_CP009273/496935‑497089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |