Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	859,587	G→A	A164T (GCA→ACA)	fsa →	fructose‑6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	859,587	0	G	A	100.0%	47.4 / NA	13	A164T (GCA→ACA)	fsa	fructose‑6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

ATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGAT  >  NZ_CP009273/859504‑859664                                                                                    |                                                                              aTGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGa                                                                         <  1:404691/90‑1 (MQ=255)               aGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCcg                                                           >  1:152262/1‑90 (MQ=255)                    cATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCa                                                      <  2:584027/90‑1 (MQ=255)                         aGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGgcgc                                                 <  1:496850/90‑1 (MQ=255)                           aCTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTg                                               >  2:180262/1‑90 (MQ=255)                                        tACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTgg                                  >  2:334538/1‑90 (MQ=255)                                          cacCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCa                                <  1:305300/90‑1 (MQ=255)                                               gTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGAtg                           >  1:75335/1‑90 (MQ=255)                                               gTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGAtg                           >  2:372116/1‑90 (MQ=255)                                                             atgcGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTActc             <  2:21885/90‑1 (MQ=255)                                                                cgccgcAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGc          <  1:297624/90‑1 (MQ=255)                                                                   cgcAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCAc       <  1:130562/90‑1 (MQ=255)                                                                        gCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGAt  <  1:421656/90‑1 (MQ=255)                                                                                    |                                                                              ATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGAT  >  NZ_CP009273/859504‑859664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑