Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273890,3810AC54.5% 4.1 / 11.1 11intergenic (+29/‑66)potF/potGspermidine/putrescine ABC transporter substrate‑binding protein PotF/putrescine ABC transporter ATP‑binding subunit PotG
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCG  >  NZ_CP009273/890302‑890466
                                                                               |                                                                                     
aTCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACccgc                                                                             >  1:321389/1‑90 (MQ=255)
           gcgcCCCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGc                                                                  <  1:300481/90‑1 (MQ=255)
           gcccACCCCCGCGTGGCCAAAAGTAAAGAGCGAAAAATAACCCCAGGCCGAAGAGGCGGGGGGGGCGCCCCCCCCCCGCCGGCAATTCGc                                                                  <  2:241196/87‑1 (MQ=255)
                                 gggAAGAGCGGAAAATAATCCGCAGTCGTAGATGCGGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcaca                                            <  2:371615/88‑1 (MQ=255)
                                 gTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCCCCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcaca                                            <  1:93661/90‑1 (MQ=255)
                                                   cccGCAGTCGTAGATGCGGGAGGGGCGCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGagc                          <  1:285710/89‑1 (MQ=255)
                                                   cccGCAGTCGTAGATGCCGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGagc                          <  1:489619/89‑1 (MQ=255)
                                                         gTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGt                    >  1:41555/1‑90 (MQ=255)
                                                                   ccGGAGGGGCGCCCCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt                <  1:216113/84‑1 (MQ=255)
                                                                   ccGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt                >  2:216113/1‑84 (MQ=255)
                                                                           gcgcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCg  >  2:229045/1‑90 (MQ=255)
                                                                               |                                                                                     
ATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCG  >  NZ_CP009273/890302‑890466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑