Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,029,1160AC54.5% 9.0 / 12.8 11G175G (GGT→GGG) prfBpeptide chain release factor 2
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAT  >  NZ_CP009273/3029039‑3029202
                                                                             |                                                                                      
aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCa                                                                            >  1:205655/1‑90 (MQ=255)
                   cTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCgatga                                                         >  1:195271/1‑90 (MQ=255)
                               agccGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGa                                             >  1:33171/1‑90 (MQ=255)
                                       gcttAACCGCGGGATTTTTTGATCGTCAGGTTTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc                                     <  2:195271/87‑1 (MQ=255)
                                       gagTAATCGCGGGATTTTTTGATCGTCACGGTTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc                                     <  1:225749/88‑1 (MQ=255)
                                       gCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc                                     <  2:39797/90‑1 (MQ=255)
                                       gCGTAACCGCGGGAGTTTTTGATCGTCACGTTTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc                                     <  2:182467/90‑1 (MQ=255)
                                       gCGTAACCGCCGGAGTTTTTGTTCGTCCGGTTTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc                                     <  2:474640/90‑1 (MQ=255)
                                            cccGCCGGATTTTTTGTTCGTCACTTTTTAAACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTc                                <  2:286911/88‑1 (MQ=255)
                                                       ttttGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCa                     >  1:302710/1‑90 (MQ=255)
                                                                          aaTACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAt  >  1:458943/1‑90 (MQ=255)
                                                                             |                                                                                      
AGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAT  >  NZ_CP009273/3029039‑3029202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑