Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,381,0450GA48.0% 19.3 / 13.5 25S168F (TCT→TTT) aaeBp‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base G (13/0);  new base A (0/12);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.92e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTACAACGTTCGACGGCAAACTGCGGCGTAAG  >  NZ_CP009273/3380969‑3381127
                                                                            |                                                                                  
gagTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATc                                                                       >  1:21953/1‑90 (MQ=255)
gagTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATc                                                                       >  1:92030/1‑90 (MQ=255)
  gTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCg                                                                     >  1:180194/1‑90 (MQ=255)
     gCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCa                                                                  >  1:234115/1‑90 (MQ=255)
        ttATTTTATATTCCGCGACAAAAAACCTTTCCACCTCTCCCTCCCCTTTTGCTTTGAGCGACCGGGAAAAAAAGACCAAATCCGCCATaa                                                               <  2:338845/90‑1 (MQ=255)
          aaTTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATc                                                             >  1:111606/1‑90 (MQ=255)
          aaTTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATc                                                             >  1:87740/1‑90 (MQ=255)
             tGATATTGCGCGACCAGAAAACTTTCCAGCTCTCGATCCCCTTTTTTTTTGATCGATCGGGGAAAAAAGAGAAAATCCGCCATAATCGca                                                          <  2:174212/90‑1 (MQ=255)
             tGATATTGCGCGACCAGAAAACTTCCCAGCTCTCGATCCACTTCTTTTTTGATCGATCGGGGAAAAAAGAGAAAATCCGCCATAATCGca                                                          <  1:145621/90‑1 (MQ=255)
               atatTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGca                                                          >  2:442769/1‑88 (MQ=255)
               atatTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAAAAAAGAGCAAATCCGCCATAATCGca                                                          <  1:442769/88‑1 (MQ=255)
               atatTGCCCGCCCAGAAAACTTCCCACCTCTCGATCCCCTTCTTTTTTGATCGACCGCGGAAAAAAGAGAAAATCCGCCATAATCGcaca                                                        <  1:417461/90‑1 (MQ=255)
                ttttGCGCCACCACAAAACTTCCCACCCCCCAATCCCCTTTTTTTTTAACCGACCGGGAAAAAAAAAGAAAATCCGCCATAATCGcacac                                                       <  2:234115/88‑1 (MQ=255)
                tatTGCGCGCCCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAAAAAAGAGCAAATCCGCCATAATCGcacac                                                       <  1:155145/90‑1 (MQ=255)
                  ttGCGCGACCAAAAACCTTCCCACCTCTCGCCCCCTTTTTTTTTTGATCGACCGCGGAAAAAAGAGAAAATCCGCCATAATCGcacacac                                                     <  2:146539/90‑1 (MQ=255)
                  ttGCGCCCCCACAAAATTTCCCACCTCTCGACCCCCTTTTTTTTTGACCGACCGCGAAAAAAAGAGAAAATCCGCCATAATCGcacacac                                                     <  2:139404/90‑1 (MQ=255)
                   tGCGCGCCCAAAAACTTTTCCACCTCTCGACCCTTTTTTTTTTTGATCGACCGGGGAAAAAAGAGAAAATCCGCCATAATCGcacacaca                                                    <  2:92030/90‑1 (MQ=255)
                         aCCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCg                                              >  1:146539/1‑90 (MQ=255)
                                   tttCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGAtct                                    >  2:120962/1‑90 (MQ=255)
                                    tcccACCTCTCGACCCACTTTTTTTTTGACCGATCGGGGAAAAAAGAAAAAATCCGCCATAATCGCACACACAATACCGATAACGAtctc                                   <  2:196430/88‑1 (MQ=255)
                                         gCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTAc                              >  2:459427/1‑90 (MQ=255)
                                                          tGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTACAACGTTCGACGGctgtc             >  1:76007/1‑86 (MQ=255)
                                                          tGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTACAACGTTCGACGGCAAAc             >  1:464805/1‑90 (MQ=255)
                                                            tttGATCGACCGCGAAAAAAAGAGAAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTACAACGTTCGACGGCAAACTg           <  2:43337/90‑1 (MQ=255)
                                                                     tcgCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTACAACGTTCGACGGCAAACTGCGGCGTAAg  >  1:236228/1‑90 (MQ=255)
                                                                            |                                                                                  
GAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAGCAAATCCGCCATAATCGCACACACAATACCGATAACGATCTCGCTACAACGTTCGACGGCAAACTGCGGCGTAAG  >  NZ_CP009273/3380969‑3381127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑