Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,401,1660AG54.5% 5.8 / 10.3 11G67G (GGA→GGG) panFsodium/pantothenate symporter
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATA‑CGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT‑GGCTTTCAC  >  NZ_CP009273/3401087‑3401236
                                                                               |                                                                        
gCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTtata                                                                <  2:91762/90‑1 (MQ=255)
                      ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCCGGGGGGGCTTTTAAAAA‑AGGGCCGGGGTGGGGAtt                                         >  2:386518/1‑90 (MQ=255)
                           tGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATA‑Cgggc                                                      >  1:95540/1‑72 (MQ=255)
                           tGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATA‑Cgggc                                                      <  2:95540/72‑1 (MQ=255)
                               gCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATA‑CGGGCTGGGCTGGGTATTGCTGGCgat                                <  1:482533/90‑1 (MQ=255)
                                                   gTGCCAGTTCGTTTATCGGCGGGCCGGGGGATGCTTATTAATATAGGGGGGGGCGGGGGGTT‑CTGGCGGTGATTCACCTTTCTCCAGGCt            >  2:213977/1‑90 (MQ=255)
                                                   gTGCCAGTTCGTTTATCGGCGGGCCCGGGGGTGCTTATAAATA‑AGGGGTGGGGGGGGGGTTTTTGGGGGTGGTTTAGCTTCCTTCCGTCt            >  2:315056/1‑90 (MQ=255)
                                                   gTGCCAGTTCGTTTATCGGCGGGCCAGGGGCGGCTTTTAAATA‑AGGGGTGGGGTGGGGATTGCTGGGGGTGGTTTAGCTTCCTTCAGTCt            >  2:270630/1‑90 (MQ=255)
                                                     gCCAGTTCGTTTATCGGCGGGGCCGGGGGGGTTTATAAAAA‑AGGGGGGGGGGGGGGTTTGCTGGGGGTGGTTCTGCTTTCTCCAGTCT‑gg         >  2:468020/1‑90 (MQ=255)
                                                            cGTTTATCGGCGGGCCAGGAGCTGCTTATAAATA‑CGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT‑GGCTTTCAc  <  1:86983/90‑1 (MQ=255)
                                                             gTTTATCGGCGGGCCCGGGGGTGCTTTTAAATA‑AGGGGTGGGGTGGGGATTTCTGGGGGTGATTTAGCTTTCTGCCGTCTGGGCTTTcct  >  2:203605/1‑88 (MQ=255)
                                                                               |                                                                        
GCTCTATGGGCGGTATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATA‑CGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCT‑GGCTTTCAC  >  NZ_CP009273/3401087‑3401236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑