Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,964,4520TG57.1% 6.7 / 13.9 14G320G (GGT→GGG) wecBUDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing)
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACG  >  NZ_CP009273/3964368‑3964519
                                                                                    |                                                                   
cATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACggg                                                                >  1:316909/1‑89 (MQ=255)
cATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGt                                                                >  1:62081/1‑90 (MQ=255)
 aTTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACgggg                                                               >  1:326227/1‑88 (MQ=255)
   tCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCg                                                             <  2:295155/90‑1 (MQ=255)
     aGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTc                                                           <  1:62970/90‑1 (MQ=255)
     aGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTc                                                           <  2:271203/90‑1 (MQ=255)
         agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTggg                                                       >  2:6066/1‑89 (MQ=255)
         agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTGGt                                                       >  2:404833/1‑90 (MQ=255)
                           gAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCg                                     <  2:59633/90‑1 (MQ=255)
                             aaCCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGGACGGGTAAGCAGCGaa                                   >  1:4180/1‑90 (MQ=255)
                               ccTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAAtt                                 <  1:6066/90‑1 (MQ=255)
                                                          gAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGTAGGCACGGATAAACAGCGAATTGTGGGGGGAGGGACGCGGCTTTTtaaa      >  1:3173/1‑90 (MQ=255)
                                                            gCGTCCGGAAGCGGTGACGGCGGGGACGGTGGGTGTTGGAGGCCCGGCTAAGCGGCGGATTGTGGAGGGAAGGGCGCCGCTTTTTAAAGa    >  1:222890/1‑90 (MQ=255)
                                                              gTCCGGAAGCGGTGACGGCGGGTACGGGGCGGCTGGTAGGGACGGGTAAGCAGCGGAATTTTGGGGGAGGGGCGCGGCTTTTtaaaaaag  >  2:195830/1‑86 (MQ=255)
                                                                                    |                                                                   
CATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACG  >  NZ_CP009273/3964368‑3964519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑