| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,213,525 | 0 | C | T | 60.0% | ‑2.7 / 14.2 | 15 | R11K (AGA→AAA) | iclR | glyoxylate bypass operon transcriptional repressor IclR |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAA > NZ_CP009273/4213442‑4213611 | cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCcgc > 1:453391/1‑90 (MQ=255) cACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTcgcg < 1:337040/90‑1 (MQ=255) ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCgggga > 1:12581/1‑88 (MQ=255) aTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTg < 2:3458/90‑1 (MQ=255) aCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCGGGGATGGGGGGGACCCTGAc > 1:92950/1‑90 (MQ=255) cTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGGATGGGGGGGGCCATGACAGTCTCtttttt > 2:198798/1‑90 (MQ=255) tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATGGGGGGGGCCCTGACAGTCTCttttttt > 2:183613/1‑89 (MQ=255) gCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtc < 1:134479/90‑1 (MQ=255) tGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGAATGGGGGGGACCATGACAGTCTCTTTTTTTTGTATCGTGGAAAtcat > 1:29398/1‑90 (MQ=255) cGGTGGCAACGGCGGGTTTTTTGCCGCGGTTGGCGGGGAGGGGGGGGGGCCCGGCAGTGTCTTTTTTTTTTATCGTGGGAAtcattttct > 2:339733/1‑89 (MQ=255) ggCAACGGCGGGTTTTTTGCCGCGGTTTGCGGGGATGGGGGGGGCCATGACAGGCTCTTTTTTTTTTATTGTGGGAATcatttttatttt > 2:134479/1‑90 (MQ=255) aaCGGCGGGGTTTTTGCCGCGTTTCGCGGGGAAGGGGGGGGCCATGACAGGCTCTTTTTTTTTTTTTGTGGGAAACatttttattttttt > 2:128214/1‑88 (MQ=255) ggcggGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt < 1:387333/90‑1 (MQ=255) ggcggGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt < 1:431900/90‑1 (MQ=255) tttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa < 1:289889/90‑1 (MQ=255) | CGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAA > NZ_CP009273/4213442‑4213611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |