| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,403,074 | 0 | T | G | 53.3% | 9.0 / 17.7 | 16 | L77R (CTG→CGG) | yjfC | glutathionylspermidine synthase family protein |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base G (0/8); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGATCGAAAAACCGACTGCGGAACTGCATCAGATGTGCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGACGGAT > NZ_CP009273/4402985‑4403149 | gCAGATCGAAAAACCGACTGCGGAACTGCATCAGATGTGCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACt > 1:344680/1‑90 (MQ=255) cGACTGCGAAACTGCATCAGATGTGCCTGGGGGTGGTGGCTCGGGGGAAAAAAGATGAAGAGACCCTGACGCAACGGGCGATCCCGCCGt < 2:262870/90‑1 (MQ=255) cGACTGCGAAACGCCATAAAAGGTGCATTGGGGTGGGGAATCGAGCGGTAAAAGATGAAGAGTTCCGGACGAATCGGGCGACCCCGCCGt < 1:356451/90‑1 (MQ=255) gCGAAACTGAATAAGATGCCCTTGGGGGTGGGGGATGGCGGGGAAAAAGATAAAGAGCCCCTGCCCCACCGGGCGATCCCGCCGTTGTAc < 2:7390/90‑1 (MQ=255) gggACCTGCATCAGATGTGCTTTGGGGTGGTGGATCGCGGGGAAAAAGATGAAGAGATCCTGACGAAACGGGCGATCCCGCCGTTGTACt < 1:334993/89‑1 (MQ=255) gCATCAGATGTGCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGAtgt > 1:7390/1‑90 (MQ=255) aGATGTGCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCg > 2:18896/1‑90 (MQ=255) tgtgCTTGGGGGGGGGGGACCGCGGGGAAAAAGATGAAGAGACCCTGACGCAACGGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTg < 2:115413/90‑1 (MQ=255) tgCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGaa > 1:115413/1‑90 (MQ=255) gAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTgg > 2:137450/1‑90 (MQ=255) gAGGCGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACAGGCGATTCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTgg > 2:383215/1‑90 (MQ=255) ggtggATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGgcgcgc > 1:61380/1‑90 (MQ=255) aaaaaGATGAAGAGATCGTGACGAAACGGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGc < 2:325636/89‑1 (MQ=255) aaGAGATCCTGACGCAACGGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGAc < 1:137450/90‑1 (MQ=255) gaTCCTGCCGCAACGGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGACggat < 1:427500/90‑1 (MQ=255) gACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGAc > 2:99575/1‑80 (MQ=255) gACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGAc < 1:99575/80‑1 (MQ=255) | GCAGATCGAAAAACCGACTGCGGAACTGCATCAGATGTGCCTTGAGGTGGTGGATCGCGCGGTAAAAGATGAAGAGATCCTGACGCAACTGGCGATCCCGCCGTTGTACTGGGATGTGATCGCTGAAAGCTGGCGCGCCCGCGATCCTTCGCTGTATGGACGGAT > NZ_CP009273/4402985‑4403149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |