| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 349,999 | 0 | T | G | 43.6% | 81.7 / 19.7 | 55 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/29); new base G (24/0); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC > NZ_CP009273/349915‑350088 | ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAg > 1:322917/1‑90 (MQ=255) gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt < 1:431724/90‑1 (MQ=255) cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc < 2:386618/90‑1 (MQ=255) gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCt > 1:111164/1‑90 (MQ=255) gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCt > 2:159481/1‑90 (MQ=255) tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑Gaa < 1:394745/90‑1 (MQ=255) tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGc < 1:29042/90‑1 (MQ=255) tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCT‑GAAAaggg > 1:170685/1‑89 (MQ=255) ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCT‑GGAAgggga > 2:454937/1‑88 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcgc < 2:48061/74‑1 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATgcgc > 1:48061/1‑74 (MQ=255) gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAgggggg > 1:154604/1‑89 (MQ=255) cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcggt < 2:314025/90‑1 (MQ=255) agagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcggt < 1:108657/89‑1 (MQ=255) aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGgtggcggtggcg < 1:56321/90‑1 (MQ=255) gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGGGGGGGGAAAGCGGCGCT‑GTAAGGGCGgggggggggggctgtt > 1:475010/1‑86 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTc < 2:91616/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:368132/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:244265/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcgc < 2:384192/59‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCAt < 2:185066/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAGAGGCGCC‑GTAAGGGGGGGAGGGGGGGGGGGTTGTcgt > 2:458556/1‑88 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGTGAAAGATgcgc > 1:384192/1‑59 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCT‑GAAAAGGCAGGGGGCGGGGGGGGTTGTCAt > 2:28646/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGAAGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCAt > 2:75908/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTcct > 2:108657/1‑88 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGa > 1:64194/1‑90 (MQ=255) ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa < 1:454937/90‑1 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag < 2:72474/90‑1 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag < 2:241005/90‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGTGGGGTTTGTCATTCCGAAagag > 1:451786/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCGAAagag > 1:494017/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGAAGCGGTGGAAAGGGCGGGGGGGGGGGGGGTTGGCATTTCCGaagaa > 1:247102/1‑87 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCT‑GAAAGGGCGGGGGGGGGGGGGGTTTGTTCTTCCCAAaaag > 1:347745/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAagagaggg > 2:410338/1‑88 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGGT‑GAAAGGGGAGGGGGGGGGGGGGGTTTTCATTTCGGAagagaggg > 1:282376/1‑88 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCg > 1:253589/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCC‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCTTTTCGAAaaaggggg > 2:307140/1‑86 (MQ=255) tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGCGGGGGGGTTTGTCATTCCGAAAAAGAGCGa > 1:263945/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:170685/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:390291/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:4850/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:399000/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:322917/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:410338/90‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 1:322409/90‑1 (MQ=255) tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa < 1:35370/90‑1 (MQ=255) tGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGGGACAGGCTggg > 2:322409/1‑89 (MQ=255) tGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGAAAGAGGGGGACAGGCTGGa > 2:390291/1‑90 (MQ=255) gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa < 1:355552/90‑1 (MQ=255) gAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt < 2:451786/90‑1 (MQ=255) gAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt < 1:307140/90‑1 (MQ=255) ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 2:235151/90‑1 (MQ=255) gggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGGGCGACAGTCTGGGAGATCGTGATGTGGCGCCCt > 2:449071/1‑90 (MQ=255) gTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg < 2:39929/90‑1 (MQ=255) tGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc > 1:340201/1‑90 (MQ=255) | TTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC > NZ_CP009273/349915‑350088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |