| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 520,722 | 0 | T | G | 57.9% | 4.9 / 20.0 | 19 | V669G (GTA→GGA) | rhsD | rhs element protein RhsD |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (11/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAGTGAG > NZ_CP009273/520638‑520795 | aCGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGAcagc > 2:186649/1‑90 (MQ=255) cGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGAcagca > 1:500797/1‑90 (MQ=255) gctgcCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGggc < 2:500797/90‑1 (MQ=255) gcCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGGCAGCAGgggggc > 1:479171/1‑90 (MQ=38) gcCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGACAACAAgggggg > 1:132757/1‑89 (MQ=37) gACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGGGGCTTTGACGGGCGGGCGCAACg > 2:531090/1‑90 (MQ=255) gACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGCGGCTTTTACGGGCGGACGCAACg > 2:658189/1‑90 (MQ=255) aaCGAGAACGGCAGCCACAGCGTCTCCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTAt < 2:408895/90‑1 (MQ=255) aaCGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGCGGCTTTGACGGGGGGACGCAACGTTATCAtttt > 2:17560/1‑88 (MQ=255) gCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTAtgacctga < 2:518942/90‑1 (MQ=255) cTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACt > 2:78903/1‑90 (MQ=255) cTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACt > 2:586113/1‑90 (MQ=255) cTTCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGCGGATTTGACGGGGGGGGGGGAGGTTATCATTATTACCCGACCGGAAAAcc > 1:696404/1‑89 (MQ=255) ttCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTc < 1:186649/90‑1 (MQ=255) ttCAGTTACGATGCGCTGGACCGGCTGGGACAGCAGGGCGGCTTTGAAGGGGGGAGGCAAAGTTATTATTATGACCTGACCGGGAAACTc > 2:652387/1‑90 (MQ=255) gTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTcacac < 2:616791/90‑1 (MQ=255) gTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTcacac < 1:208610/90‑1 (MQ=255) aCGATGCGCTGGACCGGCTGGGACAGCAGGGCGGCTTTGACGGGCGGACGCAACGGTATCATTATGACCTGACCGGGAAAATCACACaga > 1:515144/1‑90 (MQ=255) gcgcTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAGTGAg < 2:656169/90‑1 (MQ=255) | ACGATGCTGCCGGACGTGTCATCAGCCTGACCAACGAGAACGGCAGCCACAGCGTCTTCAGTTACGATGCGCTGGACCGGCTGGTACAGCAGGGCGGCTTTGACGGGCGGACGCAACGTTATCATTATGACCTGACCGGAAAACTCACACAGAGTGAG > NZ_CP009273/520638‑520795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |