| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 585,390 | 0 | T | G | 57.1% | 11.7 / 25.8 | 21 | T341P (ACC→CCC) | nfrA | bacteriophage adsorption protein NfrA |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base G (12/0); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCA > NZ_CP009273/585305‑585478 | cGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGgggggggggggg > 2:42740/1‑88 (MQ=255) gTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGTGGGGGCgg > 1:443356/1‑89 (MQ=255) ccAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCa < 1:313161/90‑1 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc < 1:410330/90‑1 (MQ=255) cTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTg < 2:698787/90‑1 (MQ=255) cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgt < 2:144291/90‑1 (MQ=255) gTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTg < 1:723946/90‑1 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc < 1:42740/90‑1 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc < 1:220782/90‑1 (MQ=255) gggTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGCGGGTTTGCGCCGCGTCGGAGTGagcct > 1:46413/1‑86 (MQ=255) cGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCCGGTTTTTCGCTGCGGCGTACCGGCCCTCttt > 2:642512/1‑90 (MQ=255) gCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTTGCTGGGGCGTGCTTACCTTCtttt > 1:537626/1‑90 (MQ=255) ctcctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTTGCTGCGTCGTTCTTACCCTCTTTTTACACGACCGGGAGGGTggc > 1:554502/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGGGGGTTTTTCGCTGCGGCGTACTGACCCTCTTTTTAAAACACCCGGAGGGgcgcgc > 2:218013/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTTTTTTTAAAACACCGGGAGCGgcgcgc > 2:395979/1‑90 (MQ=255) ctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGTTTTTTCCCTGCGTCGTACTTACCCTCTTTTTTAAACACCCGGAGCGTCGCGcc > 1:715335/1‑90 (MQ=255) ctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCTCCGCGGCGGTCTTACCTTCTTTTTAAAACACCGGGGGGGGCGCGcc > 1:74061/1‑90 (MQ=255) ctcAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGcc < 1:725205/90‑1 (MQ=255) ttCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTTGCTGCGGCGTTCTGACCTTCTTTTTATAACACCGGGAGCGGCGCGCCCACAACATa > 1:32874/1‑90 (MQ=255) ttCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGGCGTACTGACCTTCTTTTTAAAACACCGGGAGCGGCGCGCCCacaacaaa > 2:478109/1‑88 (MQ=255) ttCATTGGCGGGGGGGGGGGCGAGGAGGTTTTTCGCTGCGTCGGGCTGACCTTCTTTTTACAACACCGGGGGCGGCGCGCCCacaacaaa > 2:232116/1‑88 (MQ=255) gTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCa < 1:427939/90‑1 (MQ=255) | CGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCA > NZ_CP009273/585305‑585478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |