Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,504,6520CA55.6% 4.1 / 13.1 9S131R (AGC→AGA) ydcRPLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (0/5);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTA  >  NZ_CP009273/1504575‑1504739
                                                                             |                                                                                       
gTTCCTTTTGCCCCGGCCTTCCCCGCCCCGCGACTTTCCCCCCTCCAACAACAAACCCGCTCGCTGGCGCAGGAAAGAAAAACCGCcaca                                                                             <  2:705621/90‑1 (MQ=255)
           cTCGGCTTTCCCCGACCCGCGCTTTTCCCCCCCCCAACAACAAACCCGCTCGCGGGGGAAGGAAAGAAAAACCGCCACAGCGATGAGCGt                                                                  <  2:642503/90‑1 (MQ=255)
                             gcgACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGcc                                                >  1:741376/1‑90 (MQ=255)
                                   ttcccccccCAAACAACAAACCCGCCCGCGGGCGCAGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGaaa                                          <  2:54627/87‑1 (MQ=255)
                                                          gctcgctGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTg                   >  1:587767/1‑90 (MQ=255)
                                                             cgcgGGCGGAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtc                <  2:236157/90‑1 (MQ=255)
                                                                 ggCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtcgtcg            >  2:654169/1‑90 (MQ=255)
                                                                    gcAGGAAAAAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTa         <  2:587767/90‑1 (MQ=255)
                                                                           aGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTa  >  2:499579/1‑90 (MQ=255)
                                                                             |                                                                                       
GTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTA  >  NZ_CP009273/1504575‑1504739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑