| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 726,192 | 0 | A | C | 57.1% | 11.7 / 15.0 | 14 | D385A (GAC→GCC) | rhsC | rhs element protein RhsC |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGAAAAAGGAA > NZ_CP009273/726104‑726280 | gAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGAc < 1:335213/90‑1 (MQ=255) cAGCTAACCCCGGCGGGCTTAAGTTCCCCGTATCAGTATGAGAAGCCCCCCCCCCCCATCCCCGACCGCCTGAACCGCCGTGAAGTCCTg < 2:457282/90‑1 (MQ=9) cccgggCAGGTTAAAGTTACACGTATCAGTAGGAGAAGGCCCCCACCCCCACCCCCGAAACCCTAAACCGCCGTGAAGTCCTGCACACGc < 1:590095/86‑1 (MQ=9) ccccGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGCCCCCATCACCATCCCCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGc < 1:3936/89‑1 (MQ=14) ccccGCCAGCCTTAAGTCACACGTATAGGTAGGAGAAGGCCCCCACCCCCACCACCGACACCCTGAACCGCCGTGAAGTCCTGCACACGc < 2:550944/89‑1 (MQ=255) tACACGTATCAGTATGAGAAGGCCCGCATCCCCATCCCCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTg < 1:437420/90‑1 (MQ=17) gaGTAGGAGAAGGCCCGCCCCCCCACCCCCGACCGCCGGACCCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGggtg < 2:474474/89‑1 (MQ=17) cattATGAGAAGGCCCCACCCACCCCCCCCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGggtg < 2:501703/87‑1 (MQ=17) agaAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGaaaa > 1:100037/1‑90 (MQ=255) agaAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGaaaa > 1:232015/1‑90 (MQ=255) aaaGGCCCCCACCCCCACCCCCGACGGCCTAAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGaaaaa < 2:232015/89‑1 (MQ=17) gACCGCATCACCATCACCGACAGCCTGAACCGCCGTGa < 1:314194/38‑1 (MQ=40) gACCGCATCACCATCACCGACAGCCTGAACCGCCGTGa > 2:314194/1‑38 (MQ=255) gACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGAAAAAGGaa > 1:544169/1‑90 (MQ=255) | GAAATCTGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAGAAGGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTCCTGCACACGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGAAAAAGGAA > NZ_CP009273/726104‑726280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |