Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273970,3750AG58.3% 6.8 / 10.2 12I201V (ATT→GTT) mukFchromosome partition protein MukF
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGC‑TTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGC  >  NZ_CP009273/970296‑970452
                                                                               |                                                                              
gCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCtg                                                                      <  1:614231/90‑1 (MQ=255)
   aCGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGGTTTCAGCtgtgg                                                                   >  2:643927/1‑89 (MQ=255)
                      cagcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGC‑TTTCGGAAACt                                               <  1:59877/90‑1 (MQ=255)
                        gcagcagGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGCGGTTTTCCGCTGTGGATTTTTTCTTTTTGGAAACtc                                              >  1:567380/1‑89 (MQ=255)
                                tGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGC‑TTTCGGAAACTTCCGGAACGc                                     <  1:189709/90‑1 (MQ=255)
                                            tCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGC‑TTTCGGAAACTTCCGGAACGCTGCGTGAATTGc                         <  2:478300/90‑1 (MQ=255)
                                              gCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTCCCCCTGTGAAATGTTGC‑TTTTGGAAACATCCGGAACGCTGCGGGGAATGCAg                       >  1:478300/1‑90 (MQ=255)
                                              gCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTCCAGCTGTGAATTTTTGC‑TTTTGGAAACTTCCGGAACGCTGCGGGAATTGCAg                       >  1:235495/1‑90 (MQ=255)
                                                         gAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGC‑TTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGa            >  1:182672/1‑90 (MQ=255)
                                                             aaaGACTGGCGGGGGGGGGTTTTCAGCTGTGAATTTTTTT‑TTTTGGGAAATTCCGGAAAGCTGCGGGGATTTGAGGGAAAGCTGGGAGCg        >  2:139268/1‑90 (MQ=255)
                                                              aaGACTGGCGGGCGGGGGTTTTCAGCTGTGAATTGTTTT‑TTTTTGAAAACTCCCGAACGCTGCGGGGATTGTAGGAGAAGCTGGGAGCgg       >  2:150567/1‑90 (MQ=255)
                                                                   tGGCGGGCGGCGGTTTCCAGCTGTGGATTGTTGC‑TTTCGGAAACTTCCGGAAAGCTGCGGGGATTGCAGGGGACGCTGGAAGCGGCAggg  >  1:137507/1‑89 (MQ=255)
                                                                               |                                                                              
GCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGC‑TTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGC  >  NZ_CP009273/970296‑970452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑