Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,141,2410CA54.5% ‑1.4 / 12.1 11N29K (AAC→AAA) yegDmolecular chaperone
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATG  >  NZ_CP009273/2141160‑2141300
                                                                                 |                                                           
tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCAc                                                     <  2:509310/90‑1 (MQ=255)
     gTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACGCATTTTCTAAAAAAAGAAAAAGACAGCAcgccgc                                                >  2:220079/1‑90 (MQ=255)
      ttttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTTCTAAAAATGGAAAAAGACAGCACgctgct                                               >  1:51753/1‑90 (MQ=255)
       tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGctgctg                                              <  2:687512/90‑1 (MQ=255)
                      gCAAACTGTTCAGTGGCGGTCATTCGTGGCGGGAAACCCCCTTTTCTAAAAAAGGAAAACGACAGCACCCCGCTGCCTTCAATGCTTTgc                               >  1:714959/1‑90 (MQ=255)
                         aaCTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCACTTTCTAAAAAAGGAAAAAGAAAGCACCCTGCTGCCTTCCAAGCTTTgcgcg                            >  2:359442/1‑90 (MQ=255)
                           cTGTTCAGTGGCGGTCATGCGTGACGGGAAAACGCATTTGTTAAAAATGGAAAAAGAAAGCACGCCCCCGCCTTCAATGCTTTTCGCGcc                          >  2:432552/1‑90 (MQ=255)
                           cTGTTCAGTGGCGGGCATGCGGGACGGTAAAACCCATTTTTTAAAAAAAGAAAAAGAAAGCACGCGGCTGCCTTCCAAGCTTTTTGCGcc                          >  2:352309/1‑90 (MQ=255)
                             gTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCaa                        >  1:521396/1‑90 (MQ=255)
                                                  aCGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAAt   <  1:220079/90‑1 (MQ=255)
                                                   cGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATg  <  2:51753/90‑1 (MQ=255)
                                                                                 |                                                           
TATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATG  >  NZ_CP009273/2141160‑2141300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑