| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,336,492 | 0 | T | G | 53.3% | 10.9 / 19.0 | 15 | T218P (ACC→CCC) | fumB | class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base G (8/0); total (13/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGC > NZ_CP009273/4336408‑4336581 | tGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCCGTACCGAGGGGACGCa > 1:204011/1‑90 (MQ=255) tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGGTGCAGTACCCAGGGGACGCATTTTTTCTA‑CGAggg > 1:388508/1‑89 (MQ=255) cAATCACAAACGCGATATGGTACGGCGGGGAGGGGGCAGTAACGAGGGGACGCAATTTTTCGG‑CGAGGGAGGTTTTTTGTTTGCCggggg > 2:123598/1‑88 (MQ=255) aaCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGGGGGGACGCATTTTTTCGA‑CGAGGGAGGTTTTTTGTTTTCCGGGGGGCACCAgg > 1:631807/1‑90 (MQ=255) gATATGGTACGGCGGGCGGGGAGCAGGACCGAGGGGACGCACTTTCTCGAGCAGGGAAGTTTTT‑AGTTTTGCCGGAGTGGGCAGGGGttt > 1:338225/1‑90 (MQ=255) atatGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGA‑CGAGGAAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGCTTTg > 1:270757/1‑90 (MQ=255) gcggGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGA‑CGAGGAAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGCTTTTGGTTCCTGGt > 1:545402/1‑90 (MQ=255) gcggGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGA‑CGAGGAAGGTTTTCAGTTTGCCGGGGGGCAGCAGGGCTTTGGTTTTCTGGt > 1:173159/1‑90 (MQ=255) ccGAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTttgttg > 1:455525/1‑90 (MQ=255) gAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTcagca < 1:654581/53‑1 (MQ=255) gAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTcagca > 2:654581/1‑53 (MQ=255) gAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTgg > 1:493032/1‑70 (MQ=255) gAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTgg < 2:493032/70‑1 (MQ=255) gAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGc > 1:371106/1‑90 (MQ=255) gggTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCag > 1:389672/1‑90 (MQ=255) tACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgc > 1:378471/1‑90 (MQ=255) tACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgc > 2:241539/1‑90 (MQ=255) | TGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGA‑CGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGC > NZ_CP009273/4336408‑4336581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |