| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,594,167 | 0 | C | T | 60.0% | ‑4.9 / 17.3 | 20 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (2/6); new base T (12/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.22e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGC > NZ_CP009273/4594081‑4594254 | ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGc > 1:118410/1‑90 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCCTgaga > 1:129441/1‑90 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCC‑TTTTTTTTTTTTCTACCCCgagaga > 2:461144/1‑88 (MQ=255) tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGa < 2:168609/90‑1 (MQ=255) ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgc < 2:338016/90‑1 (MQ=255) cTATACCAGTCAGGAGTCATTTCAGGATG‑CATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc < 1:527443/90‑1 (MQ=255) tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGGgcgc > 2:510075/1‑90 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCa > 1:269103/1‑90 (MQ=255) cAGTCAGGAGTTATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga < 2:573333/90‑1 (MQ=255) cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga < 1:578547/90‑1 (MQ=255) cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga < 2:342056/90‑1 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCagagag > 1:442197/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGAGAgg > 2:243623/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAGTTTTTTTTTTTTTTGCGTTG‑GAAGAAAGCGCAGATAggg > 2:577490/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGGC‑TTTTTTTTTTTTTTTCCCTGGGAAGTGAGGGCGGAGAggg > 2:685035/1‑90 (MQ=255) ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGGAGAGGGGGGtt > 2:176242/1‑90 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCA‑TTTTTTTTTTTTTTTCCCTGAGGAGGGGGGGCAGAGAGGGAGTAtttt > 2:415991/1‑90 (MQ=255) cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCT‑TTTTTTTTTTTTTTTCCATGAGAAGGGGGCGCAGGGGGGGGTTATCTTGCctg > 1:350563/1‑90 (MQ=255) ccATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTTAGAAGGGGGGGCAGGGGGGGGGTATTTTTCCTGACTGAAc > 2:211680/1‑90 (MQ=255) cGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTTTTTTGCCTGACTGAACTGGCGATTAAGGTTc > 1:109501/1‑90 (MQ=255) ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc < 2:182127/78‑1 (MQ=255) ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc > 1:182127/1‑78 (MQ=255) ttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc > 2:226973/1‑90 (MQ=255) ttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc < 1:461144/90‑1 (MQ=255) | TTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGC > NZ_CP009273/4594081‑4594254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |