| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 63.2% | 5.1 / 17.6 | 19 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (12/0); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGT > NZ_CP009273/535937‑536103 | tCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTc < 2:526310/90‑1 (MQ=255) cagcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTTAGGGGGGTTTTTGTTTAATTTTTCAGTCAGCcgcg > 2:79673/1‑90 (MQ=255) cagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaaa < 1:79673/90‑1 (MQ=255) aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTATGTTTAATTTTTCAGGGAGTCGCGAAAGccg > 2:354395/1‑89 (MQ=255) aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTCAATTTTTCAGGCAGCCGCGAAAGCCt > 2:101145/1‑90 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTAAGGGGGGGTTATGTTCAATTTTGCAGGCAGCCCCGAAAGCCTGTTAt > 1:705162/1‑90 (MQ=255) gCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATc < 2:466323/90‑1 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGa < 1:409421/90‑1 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAggg > 1:87167/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTCAGGa > 1:454665/1‑90 (MQ=255) aTGCCCGTCCTTGCGGCGGGGATTTTCCGGGTAAGGGGGGGTTTTGTTTAATTTTTTGGGCCGGCGCGGAAGGCTGTTTTCAGGGtttta > 2:410733/1‑88 (MQ=255) aTGCCCGTCCTTGCGGCGGGCATTTTCCCGGTAAGGGGGGGTTTTGTTCAATTTTTCAGGCAGCCGCGAAAGCCTGTTATCAGGAtttta > 2:572391/1‑88 (MQ=255) gCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGTTTTTTTTTAATTTTTCAGTCAGCCGCGGAAACCTGTTTTTAGGGTTTTAGt > 2:520931/1‑90 (MQ=255) cGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTTAGTggg > 1:183067/1‑89 (MQ=255) gTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTTTTTTTAATTTTTTCGTCAGCCGCGAAAACCCGTTATCAGGGTTTTAGTgggt > 2:597687/1‑88 (MQ=255) ggggATTCTCCGGGTTAGGGGTGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATTAGGGTTTTAGTGGGtttttttttttt > 2:441097/1‑90 (MQ=255) cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac < 2:141871/90‑1 (MQ=255) cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac > 2:349075/1‑90 (MQ=255) cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac < 1:379326/90‑1 (MQ=255) tgtgtTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGt > 2:48393/1‑90 (MQ=255) | TCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGT > NZ_CP009273/535937‑536103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |