| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,200 | 0 | A | T | 56.2% | 7.6 / 18.4 | 16 | I189F (ATC→TTC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base T (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAAGC > NZ_CP009273/760111‑760288 | aTGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCGATCCCCGGCGCTAACTTTa > 1:547057/1‑90 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGAACCGATCCCCGGCTCTCACCTTTTCGAc > 2:612775/1‑90 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGGGCCCCTCCCCGGCGCTAACCTTTTTGAcct > 1:687719/1‑88 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCATCCCCGGCCCTTACCTTTTTGACAt > 1:105260/1‑90 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGAGCCCCCCCCCGGCCCTTACTTTTTCGACAt > 1:424454/1‑90 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGCTCTAAATTTTTTTACATTTTCGAAAAGt > 2:835452/1‑90 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCCCCCCCGGGCCTTACTTTTTCGACATTCTCTCAATGt > 1:742487/1‑90 (MQ=255) ggTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTTTCGACATTTTCGGAATGTTCGAAAAAGATCCGCAg > 2:297047/1‑90 (MQ=255) gACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGc < 1:184394/90‑1 (MQ=255) ccTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGa < 2:380527/90‑1 (MQ=255) cTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGAt < 2:174927/90‑1 (MQ=255) gtgtCGGTATCGGCGGGGAACCGCTCCCCGGCCCTAACTTTTTTGACATTCTCCAAAAGTTTGAAAAAAAAACGCCGACCGCagcgagcg > 1:174927/1‑90 (MQ=255) gtCGGTATCGGCGGGGGCCCGCTCCCCGGCCCTTACTTTTTTGGCATTTTCCAAAAGTTTGAAAAAAAACCGCCCACCCAAACGATCGtg > 1:380527/1‑90 (MQ=255) tGACCCCATCCCCGGCTCTTACTTTATCGACATTCTCGAAATGTTCGAAAAAAAACCGCAGACCCAAACGGACCTGATGATCGGTgagag > 2:746093/1‑89 (MQ=255) ttATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGaagaagaag > 2:774726/1‑90 (MQ=255) tATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAagc > 2:546726/1‑90 (MQ=255) tATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAagc < 1:835451/90‑1 (MQ=255) | ATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGCTGAAGAAGAAGC > NZ_CP009273/760111‑760288 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |