| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,370,606 | 0 | A | C | 56.2% | 17.4 / 21.1 | 16 | T106P (ACC→CCC) | ycjR | sugar phosphate isomerase/epimerase |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/9); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACT > NZ_CP009273/1370520‑1370685 | gCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCt > 1:259994/1‑90 (MQ=255) cTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCtt > 2:241791/1‑90 (MQ=255) cTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCtt > 2:373326/1‑90 (MQ=255) gATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTAcc > 1:683025/1‑90 (MQ=255) gcgcTTTCTCGAAGCGCGGGCAGAAGTGGGGGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTCCCTTCCGCTTACCGCCGAt < 2:264052/90‑1 (MQ=255) ctcGAAGCGCGGGCAGAGGGGGGGGGAAAAGCCTTCGTCGTCCCGGCGGGGGGGGGCATGTTTCCCTCCCGCTTACCGCCGATGACCTcg < 1:373326/90‑1 (MQ=255) aGCGCTGGCAGAGGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCTTTTTTCCCTTCCGCTTACCGCCGATGACCTCGCcgcg < 1:331157/90‑1 (MQ=255) aGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTCCCTTCCGCTTACCGCCGATGACCTCGCcgcg < 2:388640/90‑1 (MQ=255) gCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATgg < 2:259994/90‑1 (MQ=255) ctggtcgtTCCGGCGGGGGGGGGCATGTTTCCCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtg < 1:411339/87‑1 (MQ=255) ggCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtg > 1:547195/1‑79 (MQ=255) ggCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtg < 2:547196/79‑1 (MQ=255) gCGTGGGGCATGTTTCCCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcgc < 2:683026/90‑1 (MQ=255) gCGGGGGGCTTTTTTCCCTCCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcgc < 1:288683/90‑1 (MQ=255) ggggCATGTTTCCCTCCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTAc < 1:87133/90‑1 (MQ=255) gggCATTTTTCCCTTCCCTTTCCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACt < 1:75001/90‑1 (MQ=255) | GCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACT > NZ_CP009273/1370520‑1370685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |