| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,512,405 | 0 | A | C | 62.5% | 7.7 / 16.4 | 16 | F62V (TTT→GTT) | ydcZ | DMT family transporter |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/10); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGATACTGGCGAAGACAAAAAACGATCCCAGCAAGCCAGGTATAAGTGTCCACCAGCGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATG > NZ_CP009273/1512324‑1512490 | tgttACTGGCGAAGACAAAAAACGACCCCAGCAAGCCCGGTATAAGTTTCCCCCAGCGCCCGCTGGACACCATTTCCCCAACCCCCGCCa < 1:710396/87‑1 (MQ=255) aGACAAAAAACGATCCCAGCAAGCCAGGTATAAGTGTCCACCAGCGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTaa > 1:197716/1‑90 (MQ=255) aGACAAAAAACGACCCCAGCAAGCCAGGTATAAGTGTCCACCAGCGCACGCGGGACCCCAGTTCGCCAACCCCCGCCATGCCTTGTTTaa < 1:451293/90‑1 (MQ=255) aGACAAAAAACGACCCCAGCAACCCAGGTATAAGTGTCCCCCAGCCCACGCTGGACCCCAGTTCGCCAACCCCCGCCATGCCTTGTTTaa < 1:694284/90‑1 (MQ=255) aCAAAAAACGATCCCAGCAAGCCAGGTATAAGTGTCCCCCAGCGCACGCTGGACACCAGTTCGCCAACCCCCGCCATGCCTTGTTTAAAc < 2:502436/90‑1 (MQ=255) gTATAAGTGTCCCCCAGCGCACGCTGGACCCCAGTTCCCCACCCCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACaa < 2:396762/90‑1 (MQ=255) aaGTGTCCACCAGCGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATccc > 1:377671/1‑90 (MQ=255) aGTGTCCCCCAGCGCCCGCTGGACACCAGTTCGCCAACCCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATcccc < 2:660831/90‑1 (MQ=255) gtCCACCAGCGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCccacc > 2:323648/1‑90 (MQ=255) tCCACCAGCGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCccacca > 1:335937/1‑90 (MQ=255) accaGCGCCCGCGGGACCCCAGTCCCCCAACCCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTg < 1:179820/90‑1 (MQ=255) gcACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCa > 1:156593/1‑90 (MQ=255) ccAGTCCGCCAACCCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGa < 1:401568/90‑1 (MQ=255) tCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATgaga > 2:559881/1‑90 (MQ=255) ccGCAAACCCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATgagc < 1:518898/89‑2 (MQ=255) ccAACCCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATg < 1:755425/90‑1 (MQ=255) | TGATACTGGCGAAGACAAAAAACGATCCCAGCAAGCCAGGTATAAGTGTCCACCAGCGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATG > NZ_CP009273/1512324‑1512490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |