| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,679,006 | 0 | A | C | 57.1% | 9.0 / 16.1 | 14 | D164A (GAC→GCC) | tus | DNA replication terminus site‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCC > NZ_CP009273/1678917‑1679095 | ccccACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGa > 2:412917/1‑90 (MQ=255) ggAAGGGGTGCATCGTCTTTTGCGGGGGCTGACCCCCTTTATTCCTTCCCCCCCGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCtt < 2:769245/89‑1 (MQ=255) ggTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTttggttg > 2:692131/1‑90 (MQ=255) gTGCATCGTCTTTTGCGGGGGCTGACCCCCTTTAATGCTTCCCGCCCGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 2:789294/90‑1 (MQ=255) gTGCATCGTCTTTTGCCGGGGCTGACCCCCCTTAATGCTTCCCGCACGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 2:88421/90‑1 (MQ=255) gTCTTTTGCGGGGGCTGACCCCCCTTAATGCTTCCCGCACGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCtaata < 2:32186/90‑1 (MQ=255) gCTGACCCCCTTTATTGTTTCCCGCCCGCCCACCGTTCTCCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTaa < 1:23417/90‑1 (MQ=255) ttACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 2:318813/1‑90 (MQ=255) ttACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 2:486872/1‑90 (MQ=255) ttACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 2:534444/1‑90 (MQ=255) ccccGCACGCTCCCCGTTCTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGaa < 2:502053/88‑1 (MQ=255) aCGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTg > 1:257440/1‑90 (MQ=255) cGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTgg > 2:794768/1‑90 (MQ=255) ccTCACCTTTCTCCACCCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGc < 1:297491/89‑1 (MQ=255) ccccGTTCGCCACCCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGcaca < 1:1864/88‑1 (MQ=255) aCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGcc > 1:304868/1‑90 (MQ=255) | CCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCC > NZ_CP009273/1678917‑1679095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |